CenBRAIN Neurotech Center of Excellence, School of Engineering, Westlake University, Hangzhou 310030, China.
The International Center for Polaritonics, School of Sciences, Westlake University, Hangzhou 310030, China.
Sensors (Basel). 2024 Aug 9;24(16):5153. doi: 10.3390/s24165153.
() is a common pathogen with a high prevalence of infection in human populations. The diagnosis of infection is critical for its treatment, eradication, and prognosis. Biosensors have been demonstrated to be powerful for the rapid onsite detection of pathogens, particularly for point-of-care test (POCT) scenarios. In this work, we propose a novel optical biosensor, based on nanomaterial porous silicon (PSi) and photonic surface state Tamm Plasmon Polariton (TPP), for the detection of cytotoxin-associated antigen A (CagA) of bacterium. We fabricated the PSi TPP biosensor, analyzed its optical characteristics, and demonstrated through experiments, with the sensing of the CagA antigen, that the TPP biosensor has a sensitivity of 100 pm/(ng/mL), a limit of detection of 0.05 ng/mL, and specificity in terms of positive-to-negative ratio that is greater than six. From these performance factors, it can be concluded that the TPP biosensor can serve as an effective tool for the diagnosis of infection, either in analytical labs or in POCT applications.
（）是一种常见的病原体，在人群中感染率很高。感染的诊断对于其治疗、根除和预后至关重要。生物传感器已被证明是快速现场检测病原体的有力工具，特别是在即时检测（POCT）场景中。在这项工作中，我们提出了一种基于纳米材料多孔硅（PSi）和光子表面态 Tamm 等离子体激元（TPP）的新型光学生物传感器，用于检测细菌的细胞毒素相关抗原 A（CagA）。我们制造了 PSi TPP 生物传感器，分析了它的光学特性，并通过实验证明，通过对 CagA 抗原的感应，TPP 生物传感器具有 100 pm/（ng/mL）的灵敏度、0.05 ng/mL 的检测限和大于六的阳性与阴性比值的特异性。从这些性能因素可以得出结论，TPP 生物传感器可以作为感染诊断的有效工具，无论是在分析实验室还是在 POCT 应用中。
