Iwamoto Hiroyuki, Oiwa Kazuhiro, Shiba Kogiku, Inaba Kazuo
Scattering and Imaging Division, Japan Synchrotron Radiation Research Institute, Sayo, Hyogo 679-5198, Japan.
Advanced ICT Research Institute, National Institute of Information and Communications Technology, Kobe, Hyogo 651-2492, Japan.
Biophys Physicobiol. 2024 Apr 20;21(2):e210014. doi: 10.2142/biophysico.bppb-v21.0014. eCollection 2024.
This paper describes a method for recording X-ray diffraction patterns from a small amount of fibrous protein materials while being oriented by using a micro shear-flow cell. This cell consists of two concentrically arranged glass tubes. The inner tube is stationary, while the outer one rotates at a high speed. The gap between the two tubes is about 100 μm, into which the suspension of fibrous protein materials is injected. By using synchrotron-radiation X-ray microbeams (diameter, 10 μm), clear diffraction images from oriented protein materials can be recorded. The required volume of the sample is only about 10 μl. This method can also be combined with the laser-flash photolysis of caged compounds. Examples of application of this method to the flagella of a green alga , and sperm of a tunicate are presented.
本文描述了一种用于在使用微剪切流池进行定向时记录少量纤维状蛋白质材料的X射线衍射图案的方法。该池由两个同心布置的玻璃管组成。内管固定不动，而外管高速旋转。两管之间的间隙约为100μm，将纤维状蛋白质材料的悬浮液注入其中。通过使用同步辐射X射线微束（直径10μm），可以记录来自定向蛋白质材料的清晰衍射图像。所需样品体积仅约10μl。该方法还可与笼形化合物的激光闪光光解相结合。本文给出了该方法应用于绿藻鞭毛和被囊动物精子的实例。
