Fukushima Minoru, Toyonaga Takuma, O Tahara Yuhei, Nakane Daisuke, Miyata Makoto
Graduate School of Science, Osaka Metropolitan University, Osaka 558-8585, Japan.
The OCU Advanced Research Institute for Natural Science and Technology (OCARINA), Osaka Metropolitan University, Osaka 558-8585, Japan.
Biophys Physicobiol. 2024 May 28;21(2):e210015. doi: 10.2142/biophysico.bppb-v21.0015. eCollection 2024.
is a parasitic bacterium that forms gliding machinery on the cell pole and glides on a solid surface in the direction of the cell pole. The gliding machinery consists of both internal and surface structures. The internal structure is divided into a bell at the front and chain structure extending from the bell. In this study, the internal structures prepared under several conditions were analyzed using negative-staining electron microscopy and electron tomography. The chains were constructed by linked motors containing two complexes similar to ATP synthase. A cylindrical spacer with a maximum diameter of 6 nm and a height of 13 nm, and anonymous linkers with a diameter of 0.9-8.3 nm and length of 14.7±6.9 nm were found between motors. The bell is bowl-shaped and features a honeycomb surface with a periodicity of 8.4 nm. The chains of the motor are connected to the rim of the bell through a wedge-shaped structure. These structures may play roles in the assembly and cooperation of gliding machinery units.
是一种寄生细菌，它在细胞极形成滑行机制，并在固体表面沿细胞极方向滑行。滑行机制由内部结构和表面结构组成。内部结构分为前端的钟形结构和从钟形结构延伸出的链状结构。在本研究中，使用负染色电子显微镜和电子断层扫描分析了在几种条件下制备的内部结构。这些链由包含两个类似于ATP合酶的复合物的连接马达构成。在马达之间发现了一个最大直径为6纳米、高度为13纳米的圆柱形间隔物，以及直径为0.9 - 8.3纳米、长度为14.7±6.9纳米的无名连接物。钟形结构呈碗状，具有周期性为8.4纳米的蜂窝状表面。马达的链通过楔形结构连接到钟形结构的边缘。这些结构可能在滑行机制单元的组装与协作中发挥作用。
