Cui Qi, Zhao Ruixuan, Wang Zhaoqiang, Gao Liang
Opt Lett. 2024 Sep 1;49(17):4827-4830. doi: 10.1364/OL.531757.
We present cascaded spectral light field tomography for multicolor imaging in three dimensions (3D). Building upon light field tomography, our method uses a Dove prism array and a cylindrical lens array to transform a 3D scene into one-dimensional (1D) projections. To further enhance the reconstructed image quality, we incorporate a rotating Dove prism to increase the number of projection angles and a scanning light sheet to sparsify the sample along the depth axis. The resulting 1D projections are then spectrally dispersed for parallel spectral measurements. We demonstrate the effectiveness of our system in both fluorescence and scattering microscopy applications.
我们展示了用于三维(3D)多色成像的级联光谱光场断层扫描技术。基于光场断层扫描技术,我们的方法使用道威棱镜阵列和柱面透镜阵列将3D场景转换为一维(1D)投影。为了进一步提高重建图像的质量,我们加入了一个旋转道威棱镜以增加投影角度的数量,并使用扫描光片使样品在深度轴上稀疏化。然后对得到的1D投影进行光谱色散,以进行并行光谱测量。我们在荧光和散射显微镜应用中展示了我们系统的有效性。