Department of Animal Science, Iowa State University, Ames, IA 50010, USA; Hy-Line International, Dallas Center, IA 50063, USA.
Department of Reproductive Biotechnology and Cryoconservation, National Research Institute of Animal Production, 32-083 Balice, Poland.
Poult Sci. 2024 Nov;103(11):104136. doi: 10.1016/j.psj.2024.104136. Epub 2024 Aug 7.
This study focuses on estimation of the inter and intra population genetic variability of 6 duck populations. Microsatellite loci were used to assess the genetic variation and population structure of 6 duck populations under a conservation program in Poland. DNA polymorphism was assessed using 24 microsatellite markers and 50 individuals from each population. Polymorphism information content (PIC), heterozygosity with 2 estimators of genetic differentiation (FST and GST), and Nei's standard genetic distance were calculated. The results showed that these 6 endangered duck populations showed high genetic polymorphism. Observed heterozygosity within populations ranged from 0.14 to 0.83, with the average value of 0.58. PIC within populations ranged from 0.038 (P-8 and P-9 lines) to 0.89 (LsA line). Average number of alleles in the studied populations ranged from 4.5 (KhO-1 line) to 7.3 (LsA line). Based on the results, the pairs of lines LsA: P-33 and P-8: P-9 were found to be the most related; and the most genetically distant group was KhO-1 line, which originated as a cross between Khaki Campbell with Orpington duck.
本研究重点关注 6 个鸭种群的种间和种内遗传变异估计。微卫星位点用于评估波兰保护计划下 6 个鸭种群的遗传变异和种群结构。使用 24 个微卫星标记和每个种群的 50 个个体评估 DNA 多态性。计算了多态信息含量（PIC）、2 个遗传分化估计值（FST 和 GST）的杂合度和 Nei 标准遗传距离。结果表明，这 6 个濒危鸭种群表现出高遗传多态性。种群内观察到的杂合度范围为 0.14 至 0.83，平均值为 0.58。种群内的 PIC 范围为 0.038（P-8 和 P-9 品系）至 0.89（LsA 品系）。研究种群中平均等位基因数范围为 4.5（KhO-1 品系）至 7.3（LsA 品系）。根据结果，发现 LsA：P-33 和 P-8：P-9 这对品系最为相关；KhO-1 品系是遗传上最遥远的群体，它起源于卡基·坎贝尔与奥品顿鸭的杂交。
