Peronino Christophe, Rivet Nadia, Itzhar-Baikian Nathalie, Philippe Aurélien, Joly Bérangère S, de Bezieux Joseph Roux, Martin Anne-Céline, Luneau Sophie, Veyradier Agnès, Gaussem Pascale, Gendron Nicolas, Smadja David M
Université Paris Cité, Innovative Thérapies in Haemostasis, INSERM UMR-S1140, F-75006 Paris, France.
Université Paris Cité, Innovative Thérapies in Haemostasis, INSERM UMR-S1140, F-75006 Paris, France, AP-HP, Hôpital européen Georges Pompidou, Service d'Hématologie biologique, F-75015 Paris, France.
Ann Biol Clin (Paris). 2024 Sep 19;82(4):361-375. doi: 10.1684/abc.2024.1903.
The assessment of von Willebrand factor (VWF) multimer distribution, particularly following the implantation of circulatory support devices, is a crucial parameter in hemostasis. Our study aimed to evaluate the semi-automated quantification of VWF multimers using the Sebia Hydrasys analyzer. Our analysis focused on quantifying high molecular weight, intermediate weight, and low molecular weight VWF multimers. Electrophoretic migration was performed using the Hydrasys 2 scan, and interpretation was carried out using densitometric analysis with the Phoresis software. The Hydrasys scan 2 successfully separated all the expected VWF multimer profiles based on the type of von Willebrand disease. The analysis revealed that in patients with circulatory support devices, elevated levels of plasma VWF rendered multimer migration unanalyzable using the methodology recommended by the manufacturer. Therefore, adjustment to a 100 % VWF antigenic level improved gel precision. We also suggest using as a standardized control the Cryocheck™ plasma, and have established reference values. Overall, this semi-automated, standardized, and optimized VWF multimer analysis system allows for an effective assessment of the VWF multimeric profile.
血管性血友病因子（VWF）多聚体分布的评估，尤其是在植入循环支持装置后，是止血过程中的一个关键参数。我们的研究旨在评估使用Sebia Hydrasys分析仪对VWF多聚体进行半自动定量分析。我们的分析重点是对高分子量、中分子量和低分子量VWF多聚体进行定量。使用Hydrasys 2扫描进行电泳迁移，并使用Phoresis软件通过光密度分析进行解读。Hydrasys扫描2根据血管性血友病的类型成功分离出所有预期的VWF多聚体谱。分析显示，在使用循环支持装置的患者中，血浆VWF水平升高使得按照制造商推荐的方法无法分析多聚体迁移情况。因此，将VWF抗原水平调整至100%可提高凝胶精度。我们还建议使用Cryocheck™血浆作为标准化对照，并已建立参考值。总体而言，这种半自动、标准化且经过优化的VWF多聚体分析系统能够有效评估VWF多聚体谱。
