Department of Urology, The First Affiliated Hospital of Soochow University, Suzhou, China.
Department of Pathology, The First People's Hospital of Kunshan, Suzhou, China.
Kaohsiung J Med Sci. 2024 Oct;40(10):926-933. doi: 10.1002/kjm2.12891. Epub 2024 Aug 29.
This study aimed to investigate the role of cluster of differentiation 276 (CD276) in evaluating the prognosis of clear cell renal carcinoma (ccRCC) and to build a nomogram for predicting ccRCC progression post-surgery. Using data downloaded from The Cancer Genome Atlas (TCGA) database, we constructed a Kaplan-Meier (KM) curve depicting the relationship between CD276 expression levels and the progression-free interval (PFI) in 539 ccRCC cases. We further validated this by plotting a KM curve of the relationship between CD276 expression levels and PFI in 116 ccRCC patients from our hospital. Using clinical data collected from 116 patients, we identified independent risk factors affecting postoperative PFI in patients with ccRCC through univariate and multivariate COX analyses and created a nomogram for visual representation. Both TCGA and clinical data revealed a negative correlation between the expression levels of CD276 and PFI (p < 0.05). Univariate COX analysis revealed that the prognostic nutritional index, neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte ratio, systemic inflammatory index, World Health Organization grading, tumor diameter, CD276 expression levels, T stage, and N stage were related to PFI (p < 0.05). Furthermore, multivariate COX analysis indicated that tumor diameter and CD276 expression levels were independent risk factors for postoperative PFI in patients with ccRCC (p < 0.05). The calibration curve of the established nomogram exhibited a slope close to 1, with a Hosmer-Lemeshow goodness-of-fit test result of 2.335 and a p-value of 0.311. In patients with ccRCC, a negative correlation was noted between tumor CD276 expression and PFI. The larger the tumor diameter and the higher the tumor CD276 expression level, the shorter is the PFI.
本研究旨在探讨分化簇 276（CD276）在评估透明细胞肾细胞癌（ccRCC）预后中的作用，并构建用于预测术后 ccRCC 进展的列线图。我们使用从癌症基因组图谱（TCGA）数据库下载的数据，构建了Kaplan-Meier（KM）曲线，描绘了 539 例 ccRCC 病例中 CD276 表达水平与无进展生存期（PFI）之间的关系。我们通过绘制来自我们医院的 116 例 ccRCC 患者中 CD276 表达水平与 PFI 之间的 KM 曲线进一步验证了这一点。使用从 116 例患者收集的临床数据，我们通过单变量和多变量 COX 分析确定了影响 ccRCC 患者术后 PFI 的独立危险因素，并创建了一个用于可视化表示的列线图。TCGA 和临床数据均显示 CD276 表达水平与 PFI 呈负相关（p<0.05）。单变量 COX 分析显示，预后营养指数、中性粒细胞与淋巴细胞比值、血小板与淋巴细胞比值、全身炎症指数、世界卫生组织分级、肿瘤直径、CD276 表达水平、T 分期和 N 分期与 PFI 相关（p<0.05）。此外，多变量 COX 分析表明，肿瘤直径和 CD276 表达水平是 ccRCC 患者术后 PFI 的独立危险因素（p<0.05）。建立的列线图的校准曲线斜率接近 1，Hosmer-Lemeshow 拟合优度检验结果为 2.335，p 值为 0.311。在 ccRCC 患者中，肿瘤 CD276 表达与 PFI 呈负相关。肿瘤直径越大，肿瘤 CD276 表达水平越高，PFI 越短。
