• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

PHF5A对剪接的调控对黑色素瘤细胞的存活至关重要。

Splicing control by PHF5A is crucial for melanoma cell survival.

作者信息

Meißgeier Tina, Kappelmann-Fenzl Melanie, Staebler Sebastian, Ahari Ata Jadid, Mertes Christian, Gagneur Julien, Linck-Paulus Lisa, Bosserhoff Anja Katrin

机构信息

Institute of Biochemistry, Friedrich-Alexander-University Erlangen-Nürnberg (FAU), Erlangen, Germany.

Faculty of Computer Science, Deggendorf Institute of Technology, Deggendorf, Germany.

出版信息

Cell Prolif. 2025 Feb;58(2):e13741. doi: 10.1111/cpr.13741. Epub 2024 Aug 30.

DOI:10.1111/cpr.13741
PMID:39212334
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11839196/
Abstract

Abnormalities in alternative splicing are a hallmark of cancer formation. In this study, we investigated the role of the splicing factor PHD finger protein 5A (PHF5A) in melanoma. Malignant melanoma is the deadliest form of skin cancer, and patients with a high PHF5A expression show poor overall survival. Our data revealed that an siRNA-mediated downregulation of PHF5A in different melanoma cell lines leads to massive splicing defects of different tumour-relevant genes. The loss of PHF5A results in an increased rate of apoptosis by triggering Fas- and unfolded protein response (UPR)-mediated apoptosis pathways in melanoma cells. These findings are tumour-specific because we did not observe this regulation in fibroblasts. Our study identifies a crucial role of PHF5A as driver for melanoma malignancy and the described underlying splicing network provides an interesting basis for the development of new therapeutic targets for this aggressive form of skin cancer.

摘要

可变剪接异常是癌症形成的一个标志。在本研究中，我们调查了剪接因子PHD指蛋白5A（PHF5A）在黑色素瘤中的作用。恶性黑色素瘤是最致命的皮肤癌形式，PHF5A高表达的患者总体生存率较差。我们的数据显示，在不同的黑色素瘤细胞系中，siRNA介导的PHF5A下调会导致不同肿瘤相关基因出现大量剪接缺陷。PHF5A的缺失通过触发黑色素瘤细胞中Fas和未折叠蛋白反应（UPR）介导的凋亡途径，导致凋亡率增加。这些发现具有肿瘤特异性，因为我们在成纤维细胞中未观察到这种调控。我们的研究确定了PHF5A作为黑色素瘤恶性肿瘤驱动因子的关键作用，所描述的潜在剪接网络为开发针对这种侵袭性皮肤癌的新治疗靶点提供了有趣的基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9ba/11839196/1b6e27d8f285/CPR-58-e13741-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9ba/11839196/6bb86b555335/CPR-58-e13741-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9ba/11839196/46d3eeb5c616/CPR-58-e13741-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9ba/11839196/123bca1872c0/CPR-58-e13741-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9ba/11839196/adbf8bebaae2/CPR-58-e13741-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9ba/11839196/8bab97567c03/CPR-58-e13741-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9ba/11839196/1b6e27d8f285/CPR-58-e13741-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9ba/11839196/6bb86b555335/CPR-58-e13741-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9ba/11839196/46d3eeb5c616/CPR-58-e13741-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9ba/11839196/123bca1872c0/CPR-58-e13741-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9ba/11839196/adbf8bebaae2/CPR-58-e13741-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9ba/11839196/8bab97567c03/CPR-58-e13741-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9ba/11839196/1b6e27d8f285/CPR-58-e13741-g004.jpg

相似文献

1
Splicing control by PHF5A is crucial for melanoma cell survival.PHF5A对剪接的调控对黑色素瘤细胞的存活至关重要。
Cell Prolif. 2025 Feb;58(2):e13741. doi: 10.1111/cpr.13741. Epub 2024 Aug 30.
2
PHF5A Epigenetically Inhibits Apoptosis to Promote Breast Cancer Progression.PHF5A 通过表观遗传抑制细胞凋亡促进乳腺癌进展。
Cancer Res. 2018 Jun 15;78(12):3190-3206. doi: 10.1158/0008-5472.CAN-17-3514. Epub 2018 Apr 26.
3
PHD finger protein 5A promoted lung adenocarcinoma progression via alternative splicing.PHD 指状蛋白 5A 通过可变剪接促进肺腺癌进展。
Cancer Med. 2019 May;8(5):2429-2441. doi: 10.1002/cam4.2115. Epub 2019 Apr 1.
4
The DEAD/DEAH box helicase, DDX11, is essential for the survival of advanced melanomas.DEAD/DEAH 盒解旋酶 DDX11 对晚期黑色素瘤的存活至关重要。
Mol Cancer. 2012 Nov 1;11:82. doi: 10.1186/1476-4598-11-82.
5
Comprehensive systems biology analysis reveals splicing factor contributions to cutaneous melanoma progression.综合系统生物学分析揭示剪接因子对皮肤黑色素瘤进展的作用。
Sci Rep. 2025 Mar 19;15(1):9486. doi: 10.1038/s41598-025-93695-x.
6
PHD-finger domain protein 5A functions as a novel oncoprotein in lung adenocarcinoma.PHD 指状结构域蛋白 5A 在肺腺癌中作为一种新型癌蛋白发挥作用。
J Exp Clin Cancer Res. 2018 Mar 22;37(1):65. doi: 10.1186/s13046-018-0736-0.
7
Acetylation of PHF5A Modulates Stress Responses and Colorectal Carcinogenesis through Alternative Splicing-Mediated Upregulation of KDM3A.PHF5A 的乙酰化通过剪接介导的 KDM3A 上调调节应激反应和结直肠癌发生。
Mol Cell. 2019 Jun 20;74(6):1250-1263.e6. doi: 10.1016/j.molcel.2019.04.009. Epub 2019 May 1.
8
Research progress and therapeutic prospect of PHF5A acting as a new target for malignant tumors.PHF5A 作为一种新型肿瘤治疗靶点的研究进展及前景展望。
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2022 Nov 25;51(5):647-655. doi: 10.3724/zdxbyxb-2022-0459.
9
KLF6 Gene and early melanoma development in a collagen I-rich extracellular environment.富含 I 型胶原的细胞外环境中 KLF6 基因与早期黑色素瘤的发生。
J Natl Cancer Inst. 2010 Aug 4;102(15):1131-47. doi: 10.1093/jnci/djq218. Epub 2010 Jul 21.
10
Genome-wide RNAi screens in human brain tumor isolates reveal a novel viability requirement for PHF5A.全基因组 RNAi 筛选鉴定出人脑肿瘤分离物中 PHF5A 的新存活需求。
Genes Dev. 2013 May 1;27(9):1032-45. doi: 10.1101/gad.212548.112.

引用本文的文献

1
Comprehensive systems biology analysis reveals splicing factor contributions to cutaneous melanoma progression.综合系统生物学分析揭示剪接因子对皮肤黑色素瘤进展的作用。
Sci Rep. 2025 Mar 19;15(1):9486. doi: 10.1038/s41598-025-93695-x.

本文引用的文献

1
PHF5A regulates the expression of the DOCK5 variant to promote HNSCC progression through p38 MAPK activation.PHF5A 通过激活 p38 MAPK 调节 DOCK5 变异体的表达促进头颈部鳞状细胞癌的进展。
Biol Direct. 2023 Jul 12;18(1):39. doi: 10.1186/s13062-023-00396-4.
2
Splicing Factor SRSF1 Promotes Pancreatitis and KRASG12D-Mediated Pancreatic Cancer.剪接因子 SRSF1 促进胰腺炎和 KRASG12D 介导的胰腺癌。
Cancer Discov. 2023 Jul 7;13(7):1678-1695. doi: 10.1158/2159-8290.CD-22-1013.
3
The Gene Ontology knowledgebase in 2023.2023 版基因本体论知识库。
Genetics. 2023 May 4;224(1). doi: 10.1093/genetics/iyad031.
4
Cold Atmospheric Plasma Triggers Apoptosis via the Unfolded Protein Response in Melanoma Cells.冷大气等离子体通过黑色素瘤细胞中的未折叠蛋白反应触发细胞凋亡。
Cancers (Basel). 2023 Feb 7;15(4):1064. doi: 10.3390/cancers15041064.
5
Splicing factor BUD31 promotes ovarian cancer progression through sustaining the expression of anti-apoptotic BCL2L12.剪接因子 BUD31 通过维持抗凋亡 BCL2L12 的表达促进卵巢癌进展。
Nat Commun. 2022 Oct 21;13(1):6246. doi: 10.1038/s41467-022-34042-w.
6
Nuclear AREG affects a low-proliferative phenotype and contributes to drug resistance of melanoma.细胞核内的双调蛋白影响低增殖表型并导致黑色素瘤的耐药性。
Int J Cancer. 2022 Dec 15;151(12):2244-2264. doi: 10.1002/ijc.34254. Epub 2022 Sep 5.
7
Knockdown of Lamin B1 and the Corresponding Lamin B Receptor Leads to Changes in Heterochromatin State and Senescence Induction in Malignant Melanoma.敲低核膜蛋白 lamin B1 和相应的核膜蛋白受体导致恶性黑色素瘤异染色质状态改变和衰老诱导。
Cells. 2022 Jul 8;11(14):2154. doi: 10.3390/cells11142154.
8
Inositol monophosphatase 1 (IMPA1) promotes triple-negative breast cancer progression through regulating mTOR pathway and EMT process.肌醇单磷酸酶 1（IMPA1）通过调节 mTOR 通路和 EMT 过程促进三阴性乳腺癌的进展。
Cancer Med. 2023 Jan;12(2):1602-1615. doi: 10.1002/cam4.4970. Epub 2022 Jul 7.
9
The Effects of Hedgehog Signaling Pathway on the Proliferation and Apoptosis of Melanoma Cells.刺猬信号通路对黑色素瘤细胞增殖和凋亡的影响
J Oncol. 2022 Jan 4;2022:4984866. doi: 10.1155/2022/4984866. eCollection 2022.
10
PHF5A promotes colorectal cancerprogression by alternative splicing of TEAD2.PHF5A通过TEAD2的可变剪接促进结直肠癌进展。
Mol Ther Nucleic Acids. 2021 Nov 3;26:1215-1227. doi: 10.1016/j.omtn.2021.10.025. eCollection 2021 Dec 3.