Chapekar M S, Glazer R I
Cancer Res. 1985 Jun;45(6):2539-44.
The cytocidal activity of human immune interferon (IFN-gamma) in combination with the synthetic double-stranded RNA, poly(I).poly(C), was investigated in human colon carcinoma cell line HT-29. Three days of treatment with IFN-gamma (10 to 25 units/ml) resulted in 30 to 40% reduction in colony formation, whereas poly(I).poly(C) (25 to 100 micrograms/ml) reduced cell viability by 10 to 20% of control. The lethal effect of the combination of IFN-gamma and poly(I).poly(C) was synergistic wherein 70 to 90% reduction in colony formation was observed. Measurements of DNA, RNA, and protein synthesis after IFN-gamma and poly(I).poly(C) treatment showed a dose-dependent reduction in all three parameters. Recombinant IFN-gamma in combination with poly(I).poly(C) exhibited a similar effect. Studies evaluating the molecular mechanism of IFN-gamma and poly(I).poly(C) toxicity indicate a lack of involvement of the double-stranded RNA-dependent (2',5')oligoadenylate-RNase L and protein kinase pathways; however, the effect appears to be related to the inhibition of ribosomal RNA transcription in this cell line.
在人结肠癌细胞系HT - 29中研究了人免疫干扰素（IFN - γ）与合成双链RNA聚肌苷酸 - 聚胞苷酸（poly(I).poly(C)）联合使用时的杀细胞活性。用IFN - γ（10至25单位/毫升）处理三天导致集落形成减少30%至40%，而poly(I).poly(C)（25至100微克/毫升）使细胞活力比对照降低10%至20%。IFN - γ与poly(I).poly(C)联合使用的致死作用具有协同性，其中观察到集落形成减少70%至90%。IFN - γ和poly(I).poly(C)处理后对DNA、RNA和蛋白质合成的测量显示这三个参数均呈剂量依赖性降低。重组IFN - γ与poly(I).poly(C)联合使用表现出类似的效果。评估IFN - γ和poly(I).poly(C)毒性分子机制的研究表明，双链RNA依赖性（2',5'）寡聚腺苷酸 - RNase L和蛋白激酶途径未参与其中；然而，这种作用似乎与该细胞系中核糖体RNA转录的抑制有关。
