Department of Food Innovation and Health, Kyung Hee University, Gyeonggi, Korea.
Department of Medical Nutrition, Kyung Hee University, Yongin, Republic of Korea.
J Med Food. 2024 Nov;27(11):1080-1091. doi: 10.1089/jmf.2024.k.0111. Epub 2024 Aug 30.
Excessive and prolonged alcohol consumption can lead to a serious health condition known as alcohol-related liver disease (ARLD). This ailment represents a significant worldwide health challenge, affecting populations across various demographics. ARLD has a multifactorial pathogenesis involving oxidative stress, inflammation, dysregulated lipid metabolism, and apoptosis. In this study, we investigated the hepatoprotective effects of L. leaf water extract (LLE) against ARLD in alcohol-treated hepatocytes and mice. LLE exhibited antioxidant and anti-inflammatory properties by enhancing antioxidant enzyme activities and suppressing proinflammatory cytokines and CYP2E1 expression in ethanol-treated hepatocytes. Moreover, LLE mitigated lipogenesis by modulating the expression of lipogenic factors in ethanol-treated hepatocytes. , LLE administration attenuated liver injury, oxidative stress, inflammation, and lipid accumulation induced by alcohol consumption in mice. Additionally, LLE suppressed apoptosis signaling pathways implicated in alcohol-induced hepatocyte apoptosis. These findings suggest that LLE functions as a multifaceted therapeutic agent for ARLD by modulating multiple cellular mechanisms, including the reduction of oxidative damage, mitigation of inflammatory responses, alleviation of lipid-mediated toxicity, and regulation of programmed cell death pathways.
过量和长期饮酒会导致一种严重的健康问题，称为酒精相关性肝病（ARLD）。这种疾病是一个全球性的重大健康挑战，影响着不同人群。ARLD 的发病机制涉及氧化应激、炎症、脂质代谢失调和细胞凋亡等多种因素。在这项研究中，我们研究了 L. 叶水提取物（LLE）对酒精处理的肝细胞和小鼠的 ARLD 的保护作用。LLE 通过增强抗氧化酶活性和抑制乙醇处理的肝细胞中促炎细胞因子和 CYP2E1 的表达，表现出抗氧化和抗炎特性。此外，LLE 通过调节乙醇处理的肝细胞中脂肪生成因子的表达来减轻脂肪生成。LLE 给药可减轻小鼠饮酒引起的肝损伤、氧化应激、炎症和脂质积累。此外，LLE 抑制了与酒精诱导的肝细胞凋亡相关的凋亡信号通路。这些发现表明，LLE 通过调节多种细胞机制，包括减少氧化损伤、减轻炎症反应、缓解脂质介导的毒性以及调节程序性细胞死亡途径，作为 ARLD 的多效治疗剂发挥作用。
