Toftgard R, Roop D R, Yuspa S H
Carcinogenesis. 1985 Apr;6(4):655-7. doi: 10.1093/carcin/6.4.655.
The level of expression of the cellular homologues to the oncogenes rasHa, rasKi, fos, myc, abl and raf was determined in chemically induced tumors, in mouse skin and in mouse epidermis after treatment with a tumor promoter. No significant increase in expression was found for any of the above proto-oncogenes. The expression of abl was reduced in both papillomas and carcinomas and after repeated applications of 12-O-tetradecanoylphorbol-13-acetate (TPA). Treatment of primary mouse epidermal cells with TPA in vitro failed to induce any marked alterations in proto-oncogene expression. Cell lines derived by treatment of epidermal cells with chemical carcinogens showed expression levels similar to untreated primary epidermal basal cells. These results suggest that increased expression of the proto-oncogenes under study is not required for tumor induction in mouse skin. The lower level of expression of abl in tumors and after repeated applications of TPA may indicate a role for this gene in some aspect of epidermal proliferation or differentiation.
在化学诱导的肿瘤、小鼠皮肤以及用肿瘤启动子处理后的小鼠表皮中，测定了与癌基因rasHa、rasKi、fos、myc、abl和raf细胞同源物的表达水平。上述任何原癌基因的表达均未发现显著增加。在乳头状瘤和癌中以及在重复应用12 - O - 十四酰佛波醇 - 13 - 乙酸酯（TPA）后，abl的表达降低。在体外，用TPA处理原代小鼠表皮细胞未能诱导原癌基因表达的任何明显改变。用化学致癌物处理表皮细胞衍生的细胞系显示出与未处理的原代表皮基底细胞相似的表达水平。这些结果表明，所研究的原癌基因表达增加并非小鼠皮肤肿瘤诱导所必需。abl在肿瘤中以及在重复应用TPA后的较低表达水平可能表明该基因在表皮增殖或分化的某些方面起作用。
