Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Istanbul University-Cerrahpasa, 34320 Avcilar, Istanbul, Türkiye.
Acta Vet Hung. 2024 Aug 30;72(3):161-168. doi: 10.1556/004.2024.01091. Print 2024 Sep 12.
ESBL-producing Escherichia coli strains threaten public health and obligate the use of last-resort antibiotics. This study identified 15 E. coli isolates through 16S rRNA and gyrB genes, specific to E. coli, in 120 egg samples (12.5%). Antibiotic resistance was detected according to the EUCAST and CLSI in E. coli isolates. 2 isolates were susceptible to all antibiotics, one isolate was resistant to one antibiotic, one isolate was resistant to 2 antibiotics, and 11 E. coli isolates (73.3%) had multidrug resistance. Most frequent antibiotic resistances were detected against ampicillin (80%), tetracycline (66.6%), and chloramphenicol (66.6%). A double-disc confirmation test was used to detect ESBL production, and blaTEM, blaSHV, blaCTX-M and blaOXA genes were searched by PCR. The blaTEM (100%) gene was found in all resistant E. coli isolates, and the blaCTX-M gene was detected in only 3 (20%) E. coli isolates. None of the E. coli isolates contained the genes responsible for carbapenem and colistin resistance. Our results show that multi-drug antibiotic resistance and the blaTEM gene are frequent in E. coli from table eggs in Istanbul. This is the first preliminary study on ESBL-producing E. coli isolates in table eggs in Türkiye.
产 ESBL 大肠杆菌菌株威胁公共健康，迫使人们不得不使用最后一线抗生素。本研究通过 16S rRNA 和gyrB 基因在 120 个鸡蛋样本（12.5%）中鉴定了 15 株大肠杆菌。根据欧盟药敏试验委员会（EUCAST）和临床实验室标准化研究所（CLSI）的标准，检测了大肠杆菌分离株的抗生素耐药性。2 株分离株对所有抗生素均敏感，1 株分离株对 1 种抗生素耐药，1 株分离株对 2 种抗生素耐药，11 株大肠杆菌分离株（73.3%）具有多药耐药性。最常见的抗生素耐药性是对氨苄西林（80%）、四环素（66.6%）和氯霉素（66.6%）的耐药性。使用双纸片确证试验检测 ESBL 产生情况，并通过 PCR 搜索 blaTEM、blaSHV、blaCTX-M 和 blaOXA 基因。所有耐药大肠杆菌分离株均检出 blaTEM（100%）基因，仅 3 株（20%）大肠杆菌分离株检出 blaCTX-M 基因。大肠杆菌分离株均未检出耐碳青霉烯类和黏菌素的基因。我们的研究结果表明，在伊斯坦布尔市售鸡蛋中的大肠杆菌中，存在多药耐药性和 blaTEM 基因。这是土耳其市售鸡蛋中产 ESBL 大肠杆菌分离株的首次初步研究。
