Wilkinson M, Morris A, Leung H, Crane I, Meager A
Eur J Immunol. 1985 Apr;15(4):404-7. doi: 10.1002/eji.1830150418.
Human interferon gamma (IFN-gamma) endogenously produced during mitogenic stimulation of human peripheral blood mononuclear cells or human T lymphocyte-enriched cultures was neutralized in situ by the addition of a polyclonal antiserum (anti-L) raised in a rabbit against partially purified natural IFN-gamma derived from peripheral blood mononuclear cells. Small decreases in mitogen-induced [3H]thymidine incorporation (lymphoproliferation) were demonstrated under these conditions. However, an antiserum (anti-G) raised in a sheep against highly purified recombinant IFN-gamma (E. coli-derived) which strongly neutralized the antiviral effect of IFN-gamma either had little effect on mitogen-induced lymphoproliferation or caused slight enhancement of mitogenesis. The interleukin 2 responsiveness of activated T lymphocytes following mitogenic stimulation was not found to be different in the presence of anti-G to that of control cultures incubated in the presence of normal sheep serum. These results suggest that IFN-gamma is not a direct requirement for lymphoproliferation.
在人外周血单个核细胞或富含人T淋巴细胞的培养物经丝裂原刺激内源性产生的人γ干扰素（IFN-γ），通过添加在兔中制备的针对部分纯化的源自外周血单个核细胞的天然IFN-γ的多克隆抗血清（抗-L）而在原位被中和。在这些条件下，丝裂原诱导的[3H]胸腺嘧啶核苷掺入（淋巴细胞增殖）有小幅下降。然而，在羊中制备的针对高度纯化的重组IFN-γ（大肠杆菌来源）的抗血清（抗-G），其能强烈中和IFN-γ的抗病毒作用，但对丝裂原诱导的淋巴细胞增殖几乎没有影响，或导致有丝分裂轻微增强。在抗-G存在的情况下，丝裂原刺激后活化T淋巴细胞的白细胞介素2反应性与在正常羊血清存在下培养的对照培养物相比，未发现有差异。这些结果表明，IFN-γ并非淋巴细胞增殖的直接必需物质。
