Chernousov M A, Metsis M L, Koteliansky V E
FEBS Lett. 1985 Apr 22;183(2):365-9. doi: 10.1016/0014-5793(85)80811-5.
Fluorescein isothiocyanate conjugated human plasma fibronectin, 70-kDa collagen-binding, 60-kDa central, 60-kDa heparin-binding, 180-kDa heparin, collagen-binding fibronectin fragments and gelatin were used to study extracellular fibronectin matrix formation. Exogenous fibronectin, gelatin, 70-kDa collagen-binding and 180-kDa heparin, collagen-binding fragments were shown to be able to bind specifically to preexisting extracellular matrix of living fibroblasts. The results suggest that: (i) Fibronectin matrix formation may occur through a self-assembly process; (ii) the NH2-terminal part of fibronectin is responsible for fibronectin-fibronectin interaction during fibronectin fibril formation; (iii) plasma fibronectin may be the source for tissue fibronectin.
异硫氰酸荧光素偶联的人血浆纤连蛋白、70 kDa 胶原结合片段、60 kDa 中央片段、60 kDa 肝素结合片段、180 kDa 肝素胶原结合纤连蛋白片段和明胶用于研究细胞外纤连蛋白基质的形成。外源性纤连蛋白、明胶、70 kDa 胶原结合片段和 180 kDa 肝素胶原结合片段被证明能够特异性结合活成纤维细胞预先存在的细胞外基质。结果表明：(i) 纤连蛋白基质的形成可能通过自组装过程发生；(ii) 纤连蛋白的 NH2 末端部分在纤连蛋白原纤维形成过程中负责纤连蛋白 - 纤连蛋白相互作用；(iii) 血浆纤连蛋白可能是组织纤连蛋白的来源。
