Univ Montpellier, Montpellier, France; UM-CNRS Laboratoire D'Informatique de Robotique et de Microelectronique de Montpellier (LIRMM), 161, Rue Ada, Montpellier, France; CNRS, Institut de Recherche en Infectiologie de Montpellier (IRIM), 1919 Route de Mende, Montpellier, France.
Univ Montpellier, Montpellier, France; CNRS, Institut de Recherche en Infectiologie de Montpellier (IRIM), 1919 Route de Mende, Montpellier, France.
Brain Stimul. 2024 Sep-Oct;17(5):1018-1023. doi: 10.1016/j.brs.2024.08.010. Epub 2024 Aug 28.
Synaptic plasticity is an essential process encoding fine-tuned brain functions, but models to study this process in adult human systems are lacking.
We aim to test whether ex vivo organotypic culture of post-mortem adult brain explants (OPABs) retain synaptic plasticity.
OPABs were seeded on 3D microelectrode arrays to measure local field potential (LFP). Paired stimulation of distant electrodes was performed over three days to investigate our capacity to modulate specific neuronal connections.
Long-term potentiation (LTP) or depression (LTD) did not occur within a single day. In contrast, after two and three days of training, OPABs showed a significant modulation of the paired electrodes' response compared to the non-paired electrodes from the same array. This response was alleviated upon treatment with dopamine.
Our work highlights that adult human brain explants retain synaptic plasticity, offering novel approaches to neural circuitry in animal-free models.
突触可塑性是编码精细脑功能的必要过程，但缺乏用于研究成人系统中这一过程的模型。
我们旨在测试死后成人脑器官型培养物（OPAB）是否保留突触可塑性。
OPAB 种植在 3D 微电极阵列上以测量局部场电位（LFP）。通过三天的远电极对刺激来研究我们调节特定神经元连接的能力。
在单个日子内不会发生长时程增强（LTP）或长时程抑制（LTD）。相比之下，经过两天和三天的训练后，OPAB 显示出与同一阵列中非配对电极相比，配对电极的反应显著调节。在用多巴胺处理后，这种反应得到缓解。
我们的工作强调了成人脑器官型培养物保留了突触可塑性，为无动物模型中的神经回路提供了新的方法。
