Faculty of Dentistry, University of Hong Kong, Hong Kong, China.
Faculty of Dentistry, University of Hong Kong, Hong Kong, China; Department of Mineralized Tissue Biology and Bioengineering, The Forsyth Institute, Cambridge, MA, USA.
J Dent. 2024 Nov;150:105320. doi: 10.1016/j.jdent.2024.105320. Epub 2024 Aug 29.
To develop a novel and biocompatible copper tetraamine fluoride (CTF) with antibacterial and nondiscolouring properties.
This study used copper fluoride and ammonia solution to develop CTF solution. The CTF was characterized by X-ray photoelectron spectroscopy (XPS). Cytotoxicity was evaluated by stem cells from human exfoliated deciduous teeth (SHED) and human gingival fibroblasts (HGF-1). The fluoride concentration was determined using ion-selective electrode. The alkalinity was measured by a pH electrode. The human dentine blocks were treated with CTF and then incubated with Streptococcus mutans to evaluate the antimicrobial and discolouring effects. The silver diamine fluoride (SDF) was employed as the positive control, and water was the negative control. The colony-forming units (CFUs) and confocal laser scanning microscopy (CLSM) were used to examine the kinetics and viability of the biofilm. The discolouring property on dentine was assessed by spectrophotometry. One-way analysis of variance with the Bonferroni post hoc test was performed to assess and compare the data.
XPS confirmed synthesis of CTF solution. The half-maximal inhibitory concentration of CTF on SHED and HGF-1 was 195±16 ppm and 137±11 ppm. The fluoride concentration was 121,000±5,000 ppm. The pH value was 9. Log CFU of the CTF, SDF and water group were 5.0 ± 0.2, 4.9 ± 0.1 and 7.4 ± 0.1 (p < 0.001, CTF, SDF<Water). CLSM showed that the dead-to-live ratio of the CTF, SDF and water group were 0.8 ± 0.2, 0.8 ± 0.2, and 0.3 ± 0.1 (p < 0.001, CTF, SDF>Water). Spectrophotometry showed that the ΔE of the CTF, SDF and water group were 5 ± 2, 6 ± 3 and 45±2 (p < 0.001, CTF, Water<SDF).
This study developed an alkaline 58% CTF solution, which is biocompatible, antibacterial and non-discolouring.
If CTF is successfully translated into clinical care, CTF can be a simple and affordable anti-caries agent for clinicians to prevent dental caries.
开发一种具有抗菌和非着色特性的新型生物相容性铜四胺氟化物（CTF）。
本研究使用氟化铜和氨溶液来开发 CTF 溶液。使用 X 射线光电子能谱（XPS）对 CTF 进行表征。通过人脱落乳牙（SHED）和成人口腔成纤维细胞（HGF-1）评估细胞毒性。使用离子选择性电极测定氟化物浓度。使用 pH 电极测量碱度。用人牙本质块处理 CTF，然后用变异链球菌孵育，以评估抗菌和着色效果。使用银氨氟化银（SDF）作为阳性对照，水作为阴性对照。使用集落形成单位（CFU）和共聚焦激光扫描显微镜（CLSM）检查生物膜的动力学和活力。使用分光光度法评估牙本质的着色特性。采用单因素方差分析和 Bonferroni 事后检验评估和比较数据。
XPS 证实了 CTF 溶液的合成。CTF 对 SHED 和 HGF-1 的半最大抑制浓度分别为 195±16 ppm 和 137±11 ppm。氟化物浓度为 121,000±5,000 ppm。pH 值为 9。CTF、SDF 和水组的 CFU 对数分别为 5.0 ± 0.2、4.9 ± 0.1 和 7.4 ± 0.1（p<0.001，CTF、SDF<Water）。CLSM 显示 CTF、SDF 和水组的死细胞与活细胞的比例分别为 0.8 ± 0.2、0.8 ± 0.2 和 0.3 ± 0.1（p<0.001，CTF、SDF>Water）。分光光度法显示 CTF、SDF 和水组的ΔE 分别为 5±2、6±3 和 45±2（p<0.001，CTF、Water<SDF）。
本研究开发了一种碱性 58% CTF 溶液，具有生物相容性、抗菌和非着色性。
如果 CTF 成功转化为临床护理，CTF 可以成为临床医生预防龋齿的简单且负担得起的抗龋剂。
