来自矿化仓鼠臼齿的碳酸酐酶的一些特性。
Some properties of carbonic anhydrase from mineralizing hamster molars.
作者信息
Dogterom A A, Wöltgens J H
出版信息
J Biol Buccale. 1985 Mar;13(1):19-25.
PMID:3921532
Abstract
Carbonic anhydrase (CA) activity in mineralizing hamster molars was determined with a radiochemical assay. Hamster molar CA appeared to be very similar to CA from other sources. No pH optimum could be determined; the KM was 13.6 mM and CA was strongly inhibited by acetazolamide. EDTA and F- had almost no effect on the CA activity. This lack of fluoride effect suggests strongly that the protective effect of fluoride on dental mineral is not mediated by CA.
摘要
用放射化学分析法测定了正在矿化的仓鼠磨牙中的碳酸酐酶（CA）活性。仓鼠磨牙中的CA似乎与其他来源的CA非常相似。无法确定最适pH值；米氏常数（KM）为13.6 mM，CA受到乙酰唑胺的强烈抑制。乙二胺四乙酸（EDTA）和氟离子（F-）对CA活性几乎没有影响。这种缺乏氟离子效应的情况强烈表明，氟对牙齿矿物质的保护作用不是由CA介导的。
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