USP13通过促进自噬相关蛋白5（ATG5）来促进急性髓系白血病细胞的增殖和自噬。
USP13 promotes acute myeloid leukemia cell proliferation and autophagy by promoting ATG5.
作者信息
Yuan Yuchu, Xue Meizhu, Zhou Feng, Gu Lifang
机构信息
Inspection Division, Changshu blood bank, Changshu, Jiangsu 215500, China.
Department of Paediatrics, Changshu NO.1 People's Hospital, Changshu, Jiangsu 215500, China.
出版信息
Tissue Cell. 2024 Dec;91:102494. doi: 10.1016/j.tice.2024.102494. Epub 2024 Aug 9.
OBJECTIVE
To elucidate the role of USP13 in acute myeloid leukemia (AML) by investigating its effects on cell growth, apoptosis and autophagy, and to explore the underlying mechanisms.
METHODS
The expression of USP13 in AML cells was assessed using quantitative PCR (qPCR) and immunoblotting. Cell Counting Kit-8 (CCK-8) and Edu staining were employed to evaluate the impact of USP13 on AML cell growth. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and immunostaining assays were conducted to examine the effects of USP13 on apoptosis and autophagy in AML cells, and immunoblot assays were performed to determine the potential underlying mechanistic pathway.
RESULTS
USP13 expression was significantly elevated in AML cells, correlating with enhanced cell proliferation and resistance to apoptosis. Moreover, USP13 promoted autophagy in AML cells. Mechanistically, USP13 was found to be associated with upregulating ATG5 expression, which promoted AML progression.
CONCLUSION
USP13 promotes AML cell growth and autophagy by upregulating ATG5.
目的
通过研究USP13对细胞生长、凋亡和自噬的影响，阐明其在急性髓系白血病（AML）中的作用，并探索潜在机制。
方法
采用定量PCR（qPCR）和免疫印迹法评估USP13在AML细胞中的表达。使用细胞计数试剂盒-8（CCK-8）和Edu染色评估USP13对AML细胞生长的影响。进行末端脱氧核苷酸转移酶dUTP缺口末端标记（TUNEL）和免疫染色试验，以检测USP13对AML细胞凋亡和自噬的影响，并进行免疫印迹试验以确定潜在的机制途径。
结果
USP13在AML细胞中的表达显著升高，与细胞增殖增强和抗凋亡能力相关。此外，USP13促进AML细胞的自噬。机制上，发现USP13与上调ATG5表达有关，这促进了AML的进展。
结论
USP13通过上调ATG5促进AML细胞生长和自噬。
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