School of Public Health, Nanjing Medical University, Nanjing 211166, China.
School of Pharmacy, Nanjing Medical University, Nanjing 211166, China.
Colloids Surf B Biointerfaces. 2024 Dec;244:114141. doi: 10.1016/j.colsurfb.2024.114141. Epub 2024 Aug 5.
Lung cancer is among most prevalent cancers in the world, in which non-small cell lung cancer (NSCLC) accounts for more than 85 % of all subtypes of lung cancers. NSCLC is often diagnosed at an advanced stage with a high mortality rate. Despite the demonstrated efficacy of chemotherapy in the treatment of NSCLC, the main drawback of current therapy is the lack of an effective drug-targeted delivery system, which may result in undesirable side effects during the clinical treatment. In this study, we construct a "dual-targeting" anti-cancer drug delivery platform by combining superparamagnetic iron oxide nanoparticles (SPIONs) with exosomes derived from NSCLC cells. We successfully promoted the targeted delivery of anti-drug doxorubicin (DOX) at the cellular levels by combining the homing targeted ability of exosomes with the magnetic targeted ability of SPIONs. Moreover, non-small cell lung cancer cell (NCI-h1299) tumor models were established. It was found that exosome-SPIONs (Exo-SPIONs) loaded with DOX exhibited optimal tumor tissue delivery and tumor suppression in the presence of an external magnetic field, and reduced the toxicity of the DOX to normal tissues. The constructed "dual-targeting" anti-cancer drug delivery platform holds promise for targeted chemotherapy for NSCLC.
肺癌是世界上最常见的癌症之一，其中非小细胞肺癌（NSCLC）占所有肺癌亚型的 85%以上。NSCLC 通常在晚期诊断，死亡率较高。尽管化疗在治疗 NSCLC 方面已被证明有效，但目前治疗的主要缺点是缺乏有效的药物靶向输送系统，这可能导致临床治疗中出现不良副作用。在这项研究中，我们通过将超顺磁性氧化铁纳米粒子（SPIONs）与源自 NSCLC 细胞的外泌体结合，构建了一种“双靶向”抗癌药物输送平台。我们成功地通过将外泌体的归巢靶向能力与 SPIONs 的磁靶向能力相结合，促进了抗药物阿霉素（DOX）在细胞水平上的靶向递送。此外，还建立了非小细胞肺癌细胞（NCI-h1299）肿瘤模型。结果发现，载有 DOX 的外泌体-SPIONs（Exo-SPIONs）在外磁场存在下表现出最佳的肿瘤组织递送和肿瘤抑制作用，并降低了 DOX 对正常组织的毒性。所构建的“双靶向”抗癌药物输送平台有望用于 NSCLC 的靶向化疗。
