Universite de Pau et Pays de l'Adour, E2S UPPA, CNRS, IPREM UMR 5254, 64000 Pau, France; MANTA - Marine Materials Research Group, Universite de Pau et des Pays de l'Adour, E2S UPPA, 64600 Anglet, France; Agronutris, R&D Department, 31650 Saint-Orens de Gameville, France.
Universite de Pau et Pays de l'Adour, E2S UPPA, CNRS, IPREM UMR 5254, 64000 Pau, France.
Carbohydr Polym. 2024 Nov 15;344:122545. doi: 10.1016/j.carbpol.2024.122545. Epub 2024 Jul 25.
The industry of insect-based proteins as feed and food products has been encountering a huge development since the last decade, and industrial-scale factories are now arising worldwide. Among all the species studied, Black Soldier Fly is one of the most promising and farmed. This rearing activity generates several by-products in the form of chitin-rich biomass that can be valorised to keep a virtuous production cycle embedded in the scope of the bioeconomy. Herein, we report the isolation of chitin and, for the first time, chitin nanocrystals (ChNCs) from all the BSF rearing by-products, i.e., moults (larval exuviae, puparium) and dead adults. Extraction yields, were dependent on the type of by-products and ranged from 5.8 % to 20.0 %, and the chemical structure of the extracts exhibited typical features of α-chitin, confirmed by FTIR, NMR, XRD and TGA analysis. Both STEM in SEM and AFM analysis confirmed the isolation of chitin nanocrystals presenting a rod-like morphology. The average nanocrystal height estimated by AFM ranged from 13 to 27 nm depending on the by-product sample. The following results highlighted the potential of BSF rearing by-products, promoting an approach to valorise those industrial waste and paving the way towards insect-based biorefinery.
自上世纪 10 年代以来，昆虫蛋白作为饲料和食品的产业一直在迅猛发展，全球范围内的工业化工厂如雨后春笋般涌现。在所有研究过的物种中，黑水虻是最有前途和养殖最多的一种。这种养殖活动会产生几种以富含几丁质的生物质形式存在的副产物，可以对其进行增值利用，以保持嵌入生物经济范畴内的良性生产循环。在此，我们首次报道了从黑水虻养殖的所有副产物（蜕皮（幼虫蜕、蛹壳）和死亡成虫）中分离出几丁质和几丁质纳米晶体（ChNCs）。提取产率取决于副产物的类型，范围从 5.8%到 20.0%，提取物的化学结构通过 FTIR、NMR、XRD 和 TGA 分析显示出典型的α-几丁质特征。SEM 中的 STEM 和 AFM 分析都证实了几丁质纳米晶体的分离，其呈现出棒状形态。通过 AFM 估计的平均纳米晶体高度取决于副产物样品，范围从 13 到 27nm。以下结果突出了黑水虻养殖副产物的潜力，促进了对这些工业废物进行增值利用的方法，并为昆虫基生物精炼铺平了道路。
