Chen Shihui, Phillips Carolyn M
Biological Sciences, University of Southern California, Los Angeles, CA, United States.
MicroPubl Biol. 2024 Aug 16;2024. doi: 10.17912/micropub.biology.001308. eCollection 2024.
Transgenes are highly susceptible to gene silencing in the germline. Here, we examine the expression of the nuclear Argonaute protein NRDE-3 , comparing two GFP::NRDE-3 strains, one constructed by bombardment and one by CRISPR. We found that the GFP::NRDE-3 strain constructed by bombardment displays transgene silencing in germline and early embryos and that NRDE-3 expression can be restored in a mutant, which disrupts the RNAi pathway. This finding reveals that NRDE-3 is not a soma-specific Argonaute protein and is, in fact, expressed in the proximal germline and early embryos.
转基因在种系中极易受到基因沉默的影响。在此，我们检测了核内AGO蛋白NRDE-3的表达，比较了两种GFP::NRDE-3菌株，一种是通过轰击构建的，另一种是通过CRISPR构建的。我们发现，通过轰击构建的GFP::NRDE-3菌株在种系和早期胚胎中表现出转基因沉默，并且在一个破坏RNAi途径的突变体中，NRDE-3的表达可以恢复。这一发现揭示了NRDE-3不是一种体细胞特异性AGO蛋白，事实上，它在近端种系和早期胚胎中表达。
