Department of Clinical Laboratory, Shenzhen Qianhai Shekou Free Trade Zone Hospital, Shenzhen, China.
Department of Pulmonary and Critical Care Medicine, Shenzhen Qianhai Shekou Free Trade Zone Hospital, Shenzhen, China.
Front Cell Infect Microbiol. 2024 Jul 30;14:1419989. doi: 10.3389/fcimb.2024.1419989. eCollection 2024.
(AB) is rising as a human pathogen of critical priority worldwide as it is the leading cause of opportunistic infections in healthcare settings and carbapenem-resistant AB is listed as a "super bacterium" or "priority pathogen for drug resistance" by the World Health Organization.
Clinical isolates of were collected and tested for antimicrobial susceptibility. Among them, carbapenem-resistant and carbapenem-sensitive were subjected to prokaryotic transcriptome sequencing. The change of sRNA and mRNA expression was analyzed by bioinformatics and validated by quantitative reverse transcription-PCR.
A total of 687 clinical isolates were collected, of which 336 strains of were resistant to carbapenem. Five hundred and six differentially expressed genes and nineteen differentially expressed sRNA candidates were discovered through transcriptomic profile analysis between carbapenem-resistant isolates and carbapenem-sensitive isolates. Possible binding sites were predicted through software for sRNA21 and , sRNA27 and , sRNA29 and , sRNA36 and , indicating a possible targeting relationship. A negative correlation was shown between sRNA21 and (r = -0.581, P = 0.007), sRNA27 and (r = -0.612, P = 0.004), sRNA29 and (r = -0.516, P = 0.020).
This study preliminarily screened differentially expressed mRNA and sRNA in carbapenem-resistant , and explored possible targeting relationships, which will help further reveal the resistance mechanism and provide a theoretical basis for the development of drugs targeting sRNA for the prevention and treatment of carbapenem-resistant infection.
（AB）作为一种全球具有关键优先级的人类病原体正在不断上升，因为它是医疗机构中机会性感染的主要原因，而且耐碳青霉烯的 AB 被世界卫生组织列为“超级细菌”或“耐药优先病原体”。
收集并测试了临床分离株的抗菌药物敏感性。其中，对耐碳青霉烯和碳青霉烯敏感的 AB 进行了原核转录组测序。通过生物信息学分析和定量逆转录-PCR 验证，分析了 sRNA 和 mRNA 表达的变化。
共收集了 687 株临床分离株，其中 336 株 AB 对碳青霉烯耐药。通过比较耐碳青霉烯分离株和碳青霉烯敏感分离株的转录组图谱分析，发现了 506 个差异表达基因和 19 个差异表达 sRNA 候选物。通过软件预测了 sRNA21 和 、sRNA27 和 、sRNA29 和 、sRNA36 和 的可能结合位点，表明存在可能的靶向关系。sRNA21 和 （r = -0.581，P = 0.007）、sRNA27 和 （r = -0.612，P = 0.004）、sRNA29 和 （r = -0.516，P = 0.020）之间呈负相关。
本研究初步筛选了耐碳青霉烯 AB 中差异表达的 mRNA 和 sRNA，并探讨了可能的靶向关系，这有助于进一步揭示其耐药机制，并为针对 sRNA 开发药物预防和治疗耐碳青霉烯 AB 感染提供理论依据。
