School of Human Settlements and Civil Engineering, Xi'an Jiaotong University, Xi'an, Shaanxi Province 710049, China.
School of Human Settlements and Civil Engineering, Xi'an Jiaotong University, Xi'an, Shaanxi Province 710049, China; College of Energy and Environmental Engineering, Hebei University of Engineering, Handan, Hebei Province 056038, China.
Sci Total Environ. 2024 Nov 20;952:175853. doi: 10.1016/j.scitotenv.2024.175853. Epub 2024 Sep 1.
Sewer pipe materials exhibit diverse inner-surface features, which can affect the attachment of biofilm and influence microbial metabolic processes. To investigate the role of the type of pipe material on the composition and metabolic capabilities of the adhering microorganisms, three sets of urban sewers (High-Density Polyethylene Pipe (HDPE), Ductile Iron Pipe (DIP), and Concrete Pipe (CP)) were constructed. Measurements of biofilm thickness and environmental factors revealed that the thickest biofilm in CP pipes reached 2000 μm, with ORP values as low as -325 mV, indicating a more suitable anaerobic microbial habitat. High-throughput sequencing showed similar relative abundances of genera related to carbon and sulfur metabolism in the DIP and CP pipes, whereas HDPE exhibited only half the relative abundance compared to that found in the other pipes. To explore the impact of pipe materials on the mechanisms of microbial response, a metagenomic approach was used to investigate the biological transformation of carbon and sulfur in wastewater. The annotations of the crucial enzyme-encoding genes related to methyl coenzyme M and sulfite reductase in DIP and CP were 50 and 110, respectively, whereas HDPE exhibited lower counts (25 and 70, respectively). This resulted in significantly lower carbon and sulfur metabolism capabilities in the HDPE biofilm than in the other two pipes. The stability of wastewater quality during the transmission process in HDPE pipes reduces the metabolic generation of toxic and harmful gases within the pipes, favoring the preservation of carbon sources for sewer systems. This study reveals the variations in carbon and sulfur metabolism in wastewater pipe systems influenced by pipe materials and provides insights for designing future sewers.
污水管道材料呈现出多样的内表面特征，这些特征会影响生物膜的附着，并影响微生物的代谢过程。为了研究管道材料类型对附着微生物组成和代谢能力的影响，构建了三组城市污水管（高密度聚乙烯管（HDPE）、球墨铸铁管（DIP）和混凝土管（CP））。生物膜厚度和环境因素的测量结果表明，CP 管中的生物膜最厚，达到 2000 μm，氧化还原电位（ORP）值低至-325 mV，表明更适合厌氧微生物栖息。高通量测序显示，DIP 和 CP 管中与碳和硫代谢相关的属的相对丰度相似，而 HDPE 的相对丰度仅为其他管中的一半。为了探究管道材料对微生物响应机制的影响，采用宏基因组学方法研究了废水中碳和硫的生物转化。DIP 和 CP 管中与甲基辅酶 M 和亚硫酸盐还原酶相关的关键酶编码基因的注释分别为 50 和 110，而 HDPE 的注释分别为 25 和 70。这导致 HDPE 生物膜的碳和硫代谢能力明显低于其他两种管道。在 HDPE 管道中，污水质量在传输过程中的稳定性降低了管道内有毒有害气体的代谢生成，有利于为污水系统保留碳源。本研究揭示了受管道材料影响的污水管道系统中碳和硫代谢的变化，并为未来污水管道的设计提供了参考。
