Comprehensive Husbandry Protocol for Catfish and Many Other Amazonian Species.

A variety of fish species have proven instrumental in the investigation of evolution, behavior, ecology, and physiology, among many other fields. Many model systems (e.g., zebrafish, guppies, and three-spined sticklebacks) have been maintained by institutions and have had protocols written with respect to their husbandry. Here we present the protocols we have developed to maintain and breed a variety of catfish species, which are native to the tropical Americas. species are excellent systems for investigating behavior, ecology, and other topics, and our husbandry protocols would be suitable for nearly every species in the genus. In addition, these protocols are appropriate for a variety of softwater Amazonian species, and we present options for a variety of housing and husbandry conditions. On the whole, we suggest that, in a scientific laboratory setting, the use of remineralized reverse osmosis water is most appropriate and that in context, a single measure, total dissolved solids, can be used to monitor the water chemistry for water introduced to fish enclosures.