Department of Oral Pathology, National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices& Beijing Key Laboratory of Digital Stomatology & NHC Key Laboratory of Digital Stomatology & NMPA Key Laboratory for Dental Materials, Peking University School and Hospital of Stomatology, No.22, Zhongguancun South Avenue, Haidian District, Beijing, 100081, PR China.
Research Unit of Precision Pathologic Diagnosis in Tumors of the Oral and Maxillofacial Regions, Chinese Academy of Medical Sciences (2019RU034), Beijing, 100081, China.
BMC Microbiol. 2024 Sep 2;24(1):318. doi: 10.1186/s12866-024-03458-3.
The role of microbes in diseases, especially cancer, has garnered significant attention. However, research on the oral microbiota in oral potentially malignant disorders (OPMDs) remains limited. Our study investigates microbial communities in OPMDs.
Oral biopsies from19 oral leukoplakia (OLK) patients, 19 proliferative verrucous leukoplakia (PVL) patients, 19 oral lichen planus (OLP) patients, and 19 oral lichenoid lesions (OLL) patients were obtained. 15 SCC specimens were also collected from PVL patients. Healthy individuals served as controls, and DNA was extracted from their paraffin-embedded tissues. 2bRAD-M sequencing generated taxonomic profiles. Alpha and beta diversity analyses, along with Linear Discriminant Analysis effect size analysis, were conducted.
Our results showed the microbial richness and diversity were significantly different among groups, with PVL-SCC resembling controls, while OLK exhibited the highest richness. Each disease group displayed unique microbial compositions, with distinct dominant bacterial species. Noteworthy alterations during PVL-SCC progression included a decline in Fusobacterium periodonticum and an elevation in Prevotella oris.
Different disease groups exhibited distinct dominant bacterial species and microbial compositions. These findings offer promise in elucidating the underlying mechanisms of this disease.
微生物在疾病中的作用，尤其是癌症，已经引起了广泛关注。然而，关于口腔潜在恶性疾病（OPMD）中的口腔微生物群的研究仍然有限。我们的研究调查了 OPMD 中的微生物群落。
从 19 例口腔白斑（OLK）患者、19 例增殖性疣状白斑（PVL）患者、19 例口腔扁平苔藓（OLP）患者和 19 例口腔类扁平苔藓病变（OLL）患者中获取口腔活检。还从 PVL 患者中收集了 15 例 SCC 标本。健康个体作为对照组，其石蜡包埋组织中提取了 DNA。2bRAD-M 测序生成分类群谱。进行了 alpha 和 beta 多样性分析，以及线性判别分析效应大小分析。
我们的结果表明，各组之间的微生物丰富度和多样性存在显著差异，PVL-SCC 类似于对照组，而 OLK 表现出最高的丰富度。每个疾病组都显示出独特的微生物组成，具有独特的优势细菌种类。在 PVL-SCC 进展过程中值得注意的变化包括牙周福赛斯坦纳氏菌的减少和普雷沃氏菌的增加。
不同的疾病组表现出不同的优势细菌种类和微生物组成。这些发现有望阐明该疾病的潜在机制。
