National Key Laboratory of Cotton Bio-breeding and Integrated Utilization /Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan, China.
Central Cotton Research Institute, Pakistan Central Cotton Committee, Multan, Pakistan.
Physiol Plant. 2024 Sep-Oct;176(5):e14497. doi: 10.1111/ppl.14497.
Climate change severely affects crop production. Cotton is one of the primary fiber crops in the world and its production is susceptible to various environmental stresses, especially drought and salinity. Development of stress tolerant genotypes is the only way to escape from these environmental constraints. We identified sixteen homologs of the Arabidopsis JUB1 gene in cotton. Expression of GhJUB1_3-At was significantly induced in the temporal expression analysis of GhJUB1 genes in the roots of drought tolerant (H177) and susceptible (S9612) cotton genotypes under drought. The silencing of the GhJUB1_3-At gene alone and together with its paralogue GhJUB1_3-Dt reduced the drought tolerance in cotton plants. The transgenic lines exhibited tolerance to the drought and salt stress as compared to the wildtype (WT). The chlorophyll and relative water contents of wildtype decreased under drought as compared to the transgenic lines. The transgenic lines showed decreased HO and increased proline levels under drought and salt stress, as compared to the WT, indicating that the transgenic lines have drought and salt stress tolerance. The expression analysis of the transgenic lines and WT revealed that GAI was upregulated in the transgenic lines in normal conditions as compared to the WT. Under drought and salt treatment, RAB18 and RD29A were strongly upregulated in the transgenic lines as compared to the WT. Conclusively, GhJUB1_3-At is not an auto activator and it is regulated by the crosstalk of GhHB7, GhRAP2-3 and GhRAV1. GhRAV1, a negative regulator of abiotic stress tolerance and positive regulator of leaf senescence, suppresses the expression of GhJUB1_3-At under severe circumstances leading to plant death.
气候变化严重影响作物生产。棉花是世界上主要的纤维作物之一，其生产易受各种环境胁迫的影响，尤其是干旱和盐胁迫。培育耐胁迫基因型是摆脱这些环境限制的唯一途径。我们在棉花中鉴定了 16 个拟南芥 JUB1 基因的同源物。在耐旱（H177）和敏感（S9612）棉花基因型根中 GhJUB1 基因的时间表达分析中，GhJUB1_3-At 的表达明显被诱导。单独沉默 GhJUB1_3-At 基因及其旁系物 GhJUB1_3-Dt 会降低棉花植株的耐旱性。与野生型（WT）相比，转基因株系表现出对干旱和盐胁迫的耐受性。与转基因株系相比，干旱下野生型的叶绿素和相对含水量降低。与 WT 相比，转基因株系在干旱和盐胁迫下表现出 HO 降低和脯氨酸水平升高，表明转基因株系具有耐旱性和耐盐性。转基因株系和 WT 的表达分析表明，与 WT 相比，正常条件下 GAI 在转基因株系中上调。在干旱和盐处理下，与 WT 相比，转基因株系中 RAB18 和 RD29A 强烈上调。总之，GhJUB1_3-At 不是自动激活子，它受 GhHB7、GhRAP2-3 和 GhRAV1 的串扰调节。GhRAV1 是一种非生物胁迫耐受性的负调节剂和叶片衰老的正调节剂，在恶劣条件下抑制 GhJUB1_3-At 的表达，导致植物死亡。
