Institute of Cytology and Genetics, School of Basic Medical Sciences, Hengyang Medical School, University of South China, Hengyang, Hunan, 421001, China.
Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou, China.
Anal Methods. 2024 Sep 26;16(37):6403-6410. doi: 10.1039/d4ay01437e.
Monkeypox, a viral zoonotic disease caused by MPXV, has emerged as a significant global health concern since the first outbreak outside Africa in 2003. As of the current data, there have been 30 189 confirmed cases of monkeypox in 88 countries, with 29 844 cases reported in 81 countries. Given the absence of prior documented instances of the disease, swift and accurate testing is imperative to contain the spread of monkeypox. In this study, we developed a LAMP detection reagent for monkeypox and evaluated its performance in terms of sensitivity, specificity, repeatability, stability, linear range, and linearity, utilizing a commercial magnetic bead-based nucleic acid extraction system. This has led to the establishment of an integrated on-site detection platform for the monkeypox virus, utilizing a closed cartridge. The sensitivity was found to be 10 copies per μL, with no cross-reactivity observed with three other viruses, indicating robust performance. The parameters of repeatability, stability, linear range, and linearity were also assessed. For 28 simulated samples, the detection results obtained from the integrated system were consistent with those from conventional laboratory methods, specifically qPCR and LAMP detection following nucleic acid extraction. The entire process can be completed in approximately one hour, making it highly suitable for immediate rapid testing.
猴痘是一种由猴痘病毒（MPXV）引起的病毒性人畜共患病，自 2003 年首次在非洲以外地区爆发以来，已成为一个重大的全球卫生关注问题。截至目前，已有 88 个国家报告了 30189 例确诊猴痘病例，其中 81 个国家报告了 29844 例。由于此前没有该疾病的记录病例，因此快速准确的检测对于控制猴痘的传播至关重要。在这项研究中，我们开发了一种用于检测猴痘的 LAMP 检测试剂，并利用商业的基于磁珠的核酸提取系统，评估了其在灵敏度、特异性、重复性、稳定性、线性范围和线性方面的性能。这导致建立了一个集成的现场检测平台，用于猴痘病毒，利用封闭的试剂盒。检测的灵敏度为 10 拷贝/μL，与另外三种病毒没有交叉反应，表明性能稳健。还评估了重复性、稳定性、线性范围和线性等参数。对于 28 个模拟样本，集成系统的检测结果与常规实验室方法（特别是 qPCR 和核酸提取后的 LAMP 检测）的结果一致。整个过程大约需要一个小时，非常适合即时快速检测。
