Myovela Hawa, Hussein Juma, Tibuhwa Donatha
Department of Molecular Biology and Biotechnology, College of Natural and Applied Sciences, University of Dar es Salaam, Dar es Salaam, Tanzania.
Nat Prod Res. 2024 Sep 3:1-9. doi: 10.1080/14786419.2024.2395492.
Endophytic fungi are useful in a variety of biological processes and may find value in pharmaceutical settings. However, there hasn't been much research done on the bioactive compounds produced by mangrove fungal endophytes from the East African coast. Our previous research revealed a significant number of mangrove endophytic fungi in Dar es Salaam, Tanzania. This study explores the antimicrobial and cytotoxic properties of these endophytic fungi. Crude extracts of 34 mangrove endophytic fungal isolates were screened, with thirteen showing antimicrobial activity against tested microorganisms. MIC and cytotoxicity tests revealed varying bioactivity. (HMD45) was particularly potent against tested organisms (MIC = <0.195 to 0.391 mg/ml) and (LC50 = 36.001). GC-MS evaluation of HMD45 extracts indicated the existence of compounds including dodecanoic acid, n-heptadecanol-1, and n-hexadecanoic acid, which may contribute to its bioactivity. These findings offer insight into the bioactivity of mangrove endophytic fungi and trigger interest for further research.
内生真菌在多种生物过程中发挥着作用，并且在制药领域可能具有价值。然而，对于东非海岸红树林内生真菌产生的生物活性化合物，尚未开展太多研究。我们之前的研究表明，坦桑尼亚达累斯萨拉姆存在大量红树林内生真菌。本研究探讨了这些内生真菌的抗菌和细胞毒性特性。对34株红树林内生真菌分离株的粗提物进行了筛选，其中13株对受试微生物表现出抗菌活性。最小抑菌浓度（MIC）和细胞毒性测试显示出不同的生物活性。（HMD45）对受试生物体具有特别强的活性（MIC = <0.195至0.39mg/ml），（半数致死浓度LC50 = 36.001）。对HMD45提取物的气相色谱 - 质谱联用（GC - MS）评估表明存在十二烷酸、正十七烷醇 - 1和正十六烷酸等化合物，这些化合物可能是其生物活性的原因。这些发现为红树林内生真菌的生物活性提供了见解，并引发了进一步研究的兴趣。
