Hubert J F, de Monti M, Duval J
C R Acad Sci III. 1985;300(16):613-6.
The biosynthesis of proteins secreted by cultured pituitary cells was studied using (35S)-methionine. The intracellular and released proteins were analyzed by polyacrylamide gel electrophoresis followed by autoradiography. The LHRH-stimulated release is accompanied by the synthesis and release of a 87,000 dalton molecule (87 K protein). This peculiar secretion is particularly evidenced when using enriched gonadotrophs obtained by centrifugal elutriation. The 87 K protein could be involved in the release process of gonadotropins.
