Department of Anatomy (LADAF), Univ. Grenoble Alpes, Domaine de La Merci, Place du commandant Nal, La Tronche, 38700, France.
Univ. Grenoble Alpes, AGEIS laboratory, Place du commandant Nal, Domaine de La Merci, La Tronche, 38700, France.
Surg Radiol Anat. 2024 Nov;46(11):1753-1760. doi: 10.1007/s00276-024-03467-x. Epub 2024 Sep 3.
To date, no non-invasive imaging modality has been employed to profile the structural intricacies of the hippocampal arterial microvasculature in humans. We hypothesised that synchrotron-based imaging of the human hippocampus would enable precise characterisation of the arterial microvasculature.
Two preserved human brains from, a 69-year-old female and a 63-year-old male body donors were imaged using hierarchical phase-contrast tomography (HiP-CT) with synchrotron radiation at multiple voxel resolutions from 25.08 μm down to 2.45 μm. Subsequent manual and semi-automatic artery segmentation were performed followed by morphometric analyses. These data were compared to published data from alternative methodologies.
HiP-CT made it possible to segment in context the arterial architecture of the human hippocampus. Our analysis identified anterior, medial and posterior hippocampal arteries arising from the P2 segment of the posterior cerebral artery on the image slices. We mapped arterial branches with external diameters greater than 50 μm in the hippocampal region. We visualised vascular asymmetry and quantified arterial structures with diameters as small as 7 μm.
Through the application of HiP-CT, we have provided the first imaging visualisation and quantification of the arterial system of the human hippocampus at high resolution in the context of whole brain imaging. Our results bridge the gap between anatomical and histological scales.
迄今为止，尚无非侵入性成像方式可用于分析人类海马体动脉微血管结构的复杂性。我们假设基于同步加速器的人海马体成像将能够精确描述动脉微血管。
使用来自 25.08μm 至 2.45μm 多个体素分辨率的基于同步加速器的分层相衬断层摄影术（HiP-CT）对来自 69 岁女性和 63 岁男性尸体供体的两个保存的人脑进行成像。随后进行手动和半自动动脉分割，然后进行形态计量分析。将这些数据与来自替代方法的已发表数据进行了比较。
HiP-CT 使我们能够在上下文环境中分割人海马体的动脉结构。我们的分析确定了发自大脑后动脉 P2 段的前、中、后海马体动脉。我们在海马区映射了外径大于 50μm 的动脉分支。我们可视化了血管不对称性，并量化了直径小至 7μm 的动脉结构。
通过应用 HiP-CT，我们在全脑成像的背景下首次以高分辨率提供了人海马体动脉系统的成像可视化和定量。我们的结果在解剖和组织学尺度之间架起了桥梁。
