嗜热四膜虫（NT-1株）抗NADPH-细胞色素c还原酶在原生动物细胞和哺乳动物肝脏微粒体中的免疫化学反应比较。

Comparison of the immunochemical responses by anti-NADPH-cytochrome c reductase of Tetrahymena pyriformis (strain NT-1) in protozoan cells and mammalian liver microsomes.

作者信息

Fukushima H, Sasaki N, Watanabe T, Kawai K, Nozawa Y

出版信息

Comp Biochem Physiol B. 1985;80(4):817-9. doi: 10.1016/0305-0491(85)90467-5.

Abstract

Comparison of the microsomal NADPH-cytochrome c reductase activities in the four Tetrahymena cells (pyriformis, strain GL and NT-1; thermophilia; ISO) and rat liver was studied. The reductase activity in strain NT-1 was lowest among four Tetrahymena cells grown at 24 degrees C. Rabbit antibody was prepared against the purified NADPH-cytochrome c reductase from Tetrahymena pyriformis (strain NT-1) microsomes. Microsomal NADPH-cytochrome c reductase activities in various Tetrahymena cells were inhibited in proportion to the amount of antibody added, in the order of GL greater than NT-1 greater than thermophilia greater than ISO. No inhibition of reductase activity by antibody was observed in rat liver microsomes.

摘要

研究了四种四膜虫细胞（梨形四膜虫、GL株和NT-1株；嗜热四膜虫；ISO）以及大鼠肝脏微粒体中微粒体NADPH-细胞色素c还原酶活性。在24℃培养的四种四膜虫细胞中，NT-1株的还原酶活性最低。制备了针对梨形四膜虫（NT-1株）微粒体中纯化的NADPH-细胞色素c还原酶的兔抗体。各种四膜虫细胞中的微粒体NADPH-细胞色素c还原酶活性按添加抗体量的比例受到抑制，顺序为GL＞NT-1＞嗜热四膜虫＞ISO。在大鼠肝脏微粒体中未观察到抗体对还原酶活性的抑制作用。