Lundberg U, Serhan C N, Samuelsson B
FEBS Lett. 1985 Jun 3;185(1):14-8. doi: 10.1016/0014-5793(85)80731-6.
The metabolism of arachidonic acid and 15-HPETE was studied in a human promyelocytic cell line (HL-60). Upon exposure to DMSO, HL-60 cells undergo differentiation and acquire a 15-lipoxygenase activity while undifferentiated cells challenged with either arachidonic acid or 15-HPETE did not enzymatically transform these precursors. Products of the arachidonic acid 15-lipoxygenase pathway were identified by HPLC. UV-absorption and gas chromatography-mass spectrometry. Results indicate that upon differentiation HL-60 cells express a 15-lipoxygenase activity as well as the ability to transform 15-HPETE to 8,15-DHETEs and 14,15-DHETE. Moreover, these findings suggest that products of the 15-lipoxygenase cascade may be generated by a single cell system.
在人早幼粒细胞系（HL-60）中研究了花生四烯酸和15-氢过氧化二十碳四烯酸（15-HPETE）的代谢。暴露于二甲基亚砜（DMSO）后，HL-60细胞发生分化并获得15-脂氧合酶活性，而用花生四烯酸或15-HPETE刺激的未分化细胞不会对这些前体进行酶促转化。通过高效液相色谱（HPLC）、紫外吸收和气相色谱-质谱联用技术鉴定了花生四烯酸15-脂氧合酶途径的产物。结果表明，HL-60细胞在分化时表达15-脂氧合酶活性以及将15-HPETE转化为8,15-二羟基二十碳四烯酸（8,15-DHETEs）和14,15-二羟基二十碳四烯酸（14,15-DHETE）的能力。此外，这些发现表明15-脂氧合酶级联反应的产物可能由单个细胞系统产生。
