Zhu Siyu, Shi Feng, Li Honghe, Ding Yiwen, Chang Wei, Ping Yuan, Song Fuqiang
Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education and Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region and Key Laboratory of Microbiology, College of Heilongjiang Province and School of Life Sciences, Heilongjiang University, Harbin, China.
Front Plant Sci. 2024 Aug 20;15:1406542. doi: 10.3389/fpls.2024.1406542. eCollection 2024.
Soil salinization is one of the major factors limiting agricultural production. Utilizing beneficial microorganisms like () to enhance plant tolerance to abiotic stresses is a highly effective method, but the influence of on the growth of soybean in natural saline-alkaline soil remains unclear. Therefore, we investigated the effects of non-inoculation, inoculation, and fertilization on the growth, antioxidant defense, osmotic adjustment, and photosynthetic gas exchange parameters of soybean under two different levels of saline-alkaline stress in non-sterilized natural saline-alkaline soil. The study found that: 1) inoculation significantly promoted soybean growth, increasing plant height, root length, and biomass. Under mildly saline-alkaline stress, the increases were 11.5%, 16.0%, and 14.8%, respectively, compared to non-inoculated treatment. Under higher stress, inoculation achieved the same level of biomass increase as fertilization, while fertilization only significantly improved stem diameter. 2) Under saline-alkaline stress, inoculation significantly increased antioxidant enzyme activities and reduced malondialdehyde (MDA) content. Under mildly stress, MDA content was reduced by 47.1% and 43.3% compared to non-inoculated and fertilized treatments, respectively. Under moderate stress, the MDA content in the inoculated group was reduced by 29.9% and 36.6% compared to non-inoculated and fertilized treatments, respectively. Fertilization only had a positive effect on peroxidase (POD) activity. 3) inoculation induced plants to produce more osmotic adjustment substances. Under mildly stress, proline, soluble sugars, and soluble proteins were increased by 345.7%, 104.4%, and 6.9%, respectively, compared to non-inoculated treatment. Under higher stress, the increases were 75.4%, 179.7%, and 12.6%, respectively. Fertilization had no significant positive effect on proline content. 4) With increasing stress, soybean photosynthetic capacity in the -inoculated treatment was significantly higher than in the non-inoculated treatment, with net photosynthetic rate increased by 14.8% and 37.0% under different stress levels. These results indicate that can enhance soybean's adaptive ability to saline-alkaline stress by regulating ROS scavenging capacity, osmotic adjustment substance content, and photosynthetic capacity, thereby promoting plant growth. This suggests that has great potential in improving soybean productivity in natural saline-alkaline soils.
土壤盐渍化是限制农业生产的主要因素之一。利用诸如（）等有益微生物来提高植物对非生物胁迫的耐受性是一种非常有效的方法，但（）对天然盐碱土壤中大豆生长的影响仍不清楚。因此，我们在未灭菌的天然盐碱土壤中，研究了在两种不同程度的盐碱胁迫下，不接种、接种（）和施肥对大豆生长、抗氧化防御、渗透调节及光合气体交换参数的影响。研究发现：1）接种（）显著促进了大豆生长，增加了株高、根长和生物量。在轻度盐碱胁迫下，与未接种处理相比，增幅分别为11.5%、16.0%和14.8%。在更高胁迫下，接种（）实现了与施肥相同水平的生物量增加，而施肥仅显著提高了茎粗。2）在盐碱胁迫下，接种（）显著提高了抗氧化酶活性，降低了丙二醛（MDA）含量。在轻度胁迫下，与未接种和施肥处理相比，MDA含量分别降低了47.1%和43.3%。在中度胁迫下，接种组的MDA含量与未接种和施肥处理相比分别降低了29.9%和3Q6%。施肥仅对过氧化物酶（POD）活性有积极影响。3）接种（）诱导植物产生更多的渗透调节物质。在轻度胁迫下，与未接种处理相比，脯氨酸、可溶性糖和可溶性蛋白分别增加了345.7%、104.4%和6.9%。在更高胁迫下，增幅分别为75.4%、179.7%和12.6%。施肥对脯氨酸含量没有显著的积极影响。4）随着胁迫加剧，接种（）处理的大豆光合能力显著高于未接种处理，在不同胁迫水平下净光合速率提高了14.8%和37.0%。这些结果表明，（）可以通过调节活性氧清除能力、渗透调节物质含量和光合能力来增强大豆对盐碱胁迫的适应能力，从而促进植物生长。这表明（）在提高天然盐碱土壤中大豆生产力方面具有巨大潜力。
