Zhang Bo, Shi Feng, Zheng Xu, Pan Hongyang, Wen Yuqiang, Song Fuqiang
Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education & Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region & Key Laboratory of Microbiology, College of Heilongjiang Province & School of Life Sciences, Heilongjiang University, Harbin, 150080, China.
Jiaxiang Industrial Technology Research Institute of Heilongjiang University, Jiaxiang, 272400, Shandong, China.
Rice (N Y). 2023 Apr 10;16(1):18. doi: 10.1186/s12284-023-00635-2.
Increased soil salinization is among the main factors that limits safe rice production. Arbuscular mycorrhizal fungi (AMF) have been shown to alleviate the toxic effects of salt stress in plants. However, more studies on AMF combined with other functional microorganisms are needed to further improve salt tolerance in rice. Therefore, the compound inoculum Funneliformis mosseae (Fm) together with two functional microorganisms, Piriformospora indica (Pi) and Agrobacterium rhizogenes (Ar) was evaluated for their effect on the rice growth, photosynthetic gas exchange parameters, ion homeostasis, and the expression of salt tolerance-related genes under 0, 80, 120 and 160 mM salt stress conditions. The results showed that: (1) the rice seedling biomass of the AMF compound inoculant treatment group was significantly higher than that of the non-inoculation treatment group (P < 0.05); (2) under NaCl stress, inoculation with AMF compound inoculants can activate the rice antioxidant enzyme system and improve osmoregulation ability; (3) AMF compound inoculants can increase the concentration of K in the plant and inhibit the transfer of Na to rice leaves, maintaining a high K/Na; and (4) AMF compound inoculants could induce and regulate the overexpression of genes related to salt tolerance, photosynthesis and ion homeostasis in rice, and improve the tolerance of rice under salt stress. Our study showed that AMF compound inoculants could improve the adaptability of rice under NaCl stress and promote plant growth by regulating the photosynthetic gas exchange parameter, reactive oxygen species (ROS) scavenging ability, and ion homeostasis of plants. These results suggest that AMF compound inoculants may play an important role in improving rice productivity in salinized soil.
土壤盐渍化加剧是限制水稻安全生产的主要因素之一。丛枝菌根真菌（AMF）已被证明可以减轻盐胁迫对植物的毒害作用。然而，需要更多关于AMF与其他功能微生物联合作用的研究，以进一步提高水稻的耐盐性。因此，评估了丛枝菌根真菌摩西斗管囊霉（Fm）与两种功能微生物印度梨形孢（Pi）和发根农杆菌（Ar）组成的复合接种剂在0、80、120和160 mM盐胁迫条件下对水稻生长、光合气体交换参数、离子稳态以及耐盐相关基因表达的影响。结果表明：（1）AMF复合接种剂处理组的水稻幼苗生物量显著高于未接种处理组（P < 0.05）；（2）在NaCl胁迫下，接种AMF复合接种剂可激活水稻抗氧化酶系统并提高渗透调节能力；（3）AMF复合接种剂可增加植物体内K的浓度，抑制Na向水稻叶片的转运，维持较高的K/Na比；（4）AMF复合接种剂可诱导并调节水稻中与耐盐性、光合作用和离子稳态相关基因的过表达，提高水稻在盐胁迫下的耐受性。我们的研究表明，AMF复合接种剂可通过调节光合气体交换参数、活性氧（ROS）清除能力和植物离子稳态来提高水稻在NaCl胁迫下的适应性并促进植物生长。这些结果表明，AMF复合接种剂可能在提高盐渍化土壤中水稻产量方面发挥重要作用。
