Michelia macclurei Dany 的生物活性成分的代谢组学分析及其抗氧化和酶抑制活性。

Metabolomics analysis of bioactive compositions of Michelia macclurei Dany and its antioxidant and enzyme inhibitory activities.

机构信息

College of Forestry, Guangxi University, Nanning, China.

Key Laboratory of National Forestry and Grassland Administration on Cultivation of Fast-Growing Timber in Central South China, College of Forestry, Guangxi University, Nanning, China.

出版信息

J Sci Food Agric. 2025 Jan 15;105(1):635-648. doi: 10.1002/jsfa.13860. Epub 2024 Sep 4.

DOI:10.1002/jsfa.13860

PMID:39230063

Abstract

BACKGROUND

Michelia macclurei Dandy is a traditional Chinese medicinal plant, but little is understood about the bioactive compositions and biological potential of its different parts, limiting their applications. This study aims to identify the bioactive compositions and analyze differences in accumulation patterns from different parts of Michelia macclurei (heartwood, sapwood, bark, root, leaf, and fruit) using metabolomics. It also seeks to explore their biological potential and analyze the relationship between the bioactive compositions and biological potential.

RESULTS

A total of 63 volatile metabolites (VMs) were identified by gas chromatography-mass spectrometry (GC-MS) in six parts, and the VMs in each part were dominated by sesquiterpenes and their derivatives (71.40-88.32%). Six parts of Michelia macclurei contained structurally diverse non-volatile metabolites (NVMs) with a total of 207 bioactive compounds, including 92 alkaloids, 30 flavonoids, 19 lignans, and 18 organic acids, utilizing ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) analysis. Multivariate statistical analysis showed that the accumulation patterns of bioactive compositions differed significantly among the different parts, and the 25 VMs and 72 NVMs could be considered potential markers for distinguishing the different parts of Michelia macclurei. The excellent antioxidant and enzyme inhibitory capacity of extracts of all six parts was indicated by in vitro bioactivity assays. Pearson's correlation analysis showed that the bioactive compositions in the six parts were significantly correlated with antioxidant and enzyme inhibitory activities.

CONCLUSION

This study offers helpful information on the distribution of bioactive compositions in different parts of Michelia macclurei and confirms the excellent antioxidant, and enzyme inhibitory potential of its extracts, which could provide scientific evidence for its potential applications in the pharmaceutical industry, cosmetics, and functional foods. © 2024 Society of Chemical Industry.

摘要

背景

含笑 Michelia macclurei Dandy 是一种传统的中药材，但对其不同部位的生物活性成分和生物潜力知之甚少，限制了其应用。本研究旨在采用代谢组学方法鉴定 Michelia macclurei（心材、边材、树皮、根、叶和果实）不同部位的生物活性成分，并分析其积累模式的差异，探讨其生物潜力，分析生物活性成分与生物潜力之间的关系。

结果

采用气相色谱-质谱联用（GC-MS）共鉴定出 63 种挥发性代谢物（VMs），各部位 VMs 以倍半萜及其衍生物为主（71.40-88.32%）。Michelia macclurei 的 6 个部位均含有结构多样的非挥发性代谢物（NVMs），共 207 种生物活性化合物，包括 92 种生物碱、30 种黄酮类化合物、19 种木脂素和 18 种有机酸，利用超高效液相色谱-质谱联用（UPLC-MS）分析。多元统计分析表明，生物活性成分的积累模式在不同部位之间存在显著差异，25 种 VMs 和 72 种 NVMs 可作为区分 Michelia macclurei 不同部位的潜在标志物。体外生物活性测定表明，所有 6 个部位提取物均具有优异的抗氧化和酶抑制能力。Pearson 相关性分析表明，6 个部位的生物活性成分与抗氧化和酶抑制活性显著相关。