Van Weemen B K
J Virol Methods. 1985 Apr;10(4):371-8. doi: 10.1016/0166-0934(85)90055-2.
ELISA is now very widely used. Many variations on the theme have been described. The search for further improvements is ongoing, mainly in the areas of sensitivity, specificity and practicability. Of course, the application of monoclonal antibodies is important in this respect. Next to that, further improvements in the detection of the enzyme label have come to the fore. Examples regarding the use of tetra-methylbenzidine as a peroxidase-substrate and details on its safety, a novel amplification method for detecting alkaline phosphatase and an enhanced luminescence method to detect peroxidase are given.
酶联免疫吸附测定法（ELISA）如今应用非常广泛。人们已经描述了许多基于该原理的变体。目前仍在继续探索进一步的改进方法，主要集中在灵敏度、特异性和实用性方面。当然，单克隆抗体的应用在这方面很重要。除此之外，酶标记检测方面也有了进一步的改进。文中给出了使用四甲基联苯胺作为过氧化物酶底物的示例及其安全性细节、一种检测碱性磷酸酶的新型放大方法以及一种增强的发光方法来检测过氧化物酶。
