The National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine, Jiangxi University of Chinese Medicine, Nanchang 330006, China.
Key Laboratory of Modern Preparation of Traditional Chinese Medicine, Ministry of Education, Jiangxi University of Chinese Medicine, Nanchang 330004, China.
J Agric Food Chem. 2024 Sep 18;72(37):20374-20382. doi: 10.1021/acs.jafc.4c01637. Epub 2024 Sep 4.
Scavenging MGO has been considered as an effective strategy for preventing atherosclerosis. A previous study showed that the total flavonoids of Apocyni Veneti Folium (TFAVF) had a significant antiatherosclerotic effect. However, there are no studies that have investigated the MGO scavenging capacities of TFAVF in mice. We found that TFAVF consisted mainly of quercetin glycosides and kaempferol glycosides using ultrahigh performance liquid chromatography coupled to quadrupole time-of-flight tandem mass spectrometry (UHPLC-QTOF-MS/MS). TFAVF was first demonstrated to effectively scavenge MGO in mice based on the formation of mono-MGO-quercetin, mono-MGO-dehydroquercetin, mono-MGO-isorhamnetin, mono-MGO-dehydroisorhamnetin, mono-MGO-kaempferol, and mono-MGO-dehydrokaempferol. In addition, one mono-MGO-quercetin was separated and purified, and its structure was elucidated as 8-MGO-quercetin based on UHPLC-QTOF-MS/MS and NMR data. Quantification studies have demonstrated that kaempferol, dehydrokaempferol, quercetin, dehydroquercetin, isorhamnetin, and dehydroisorhamnetin can dose dependently scavenge MGO in mice. Taken together, these results indicated that TFAVF showed a significant antiatherosclerotic effect, which might be based on MGO detoxification.
scavenging MGO 已被认为是预防动脉粥样硬化的有效策略。先前的研究表明，杠柳毒苷总黄酮（TFAVF）具有显著的抗动脉粥样硬化作用。然而，目前还没有研究调查 TFAVF 在小鼠体内清除 MGO 的能力。我们使用超高效液相色谱-四极杆飞行时间串联质谱联用技术（UHPLC-QTOF-MS/MS）发现，杠柳毒苷总黄酮主要由槲皮素糖苷和山柰酚糖苷组成。首次证明 TFAVF 可有效清除小鼠体内的 MGO，形成单-MGO-槲皮素、单-MGO-脱氢槲皮素、单-MGO-异鼠李素、单-MGO-脱氢异鼠李素、单-MGO-山柰酚和单-MGO-脱氢山柰酚。此外，还分离和纯化了一种单-MGO-槲皮素，并根据 UHPLC-QTOF-MS/MS 和 NMR 数据确定其结构为 8-MGO-槲皮素。定量研究表明，山柰酚、脱氢山柰酚、槲皮素、脱氢槲皮素、异鼠李素和脱氢异鼠李素可在小鼠体内剂量依赖性地清除 MGO。综上所述，这些结果表明 TFAVF 表现出显著的抗动脉粥样硬化作用，这可能基于 MGO 解毒。
