School of Pharmacy, Tokyo University of Pharmacy and Life Sciences.
Chem Pharm Bull (Tokyo). 2024;72(9):800-803. doi: 10.1248/cpb.c24-00393.
A noise filter, which is usually attached to a detector for chromatography, was applied for the improvement of a signal-to-noise ratio (S/N) on a chromatogram. The objective of this paper is to elucidate the effect of noise filtering in an UV detector of ultra HPLC (UHPLC) on the statistical reliability of chemometrically evaluated repeatability by the function of mutual information (FUMI) theory. To examine the statistical reliability of chemometrically evaluated repeatability in the UHPLC system associated with noise filtering, the standard deviation (SD) values of the area in baseline fluctuations with peak region k (s(k)) were obtained from six chromatograms with noise filtering. Further, the average of s(k) values (σ̂) was calculated from the s(k) values (n = 6) to be alternatively applied as the population SD. All s(k)/σ̂ values were within the 95% confidence intervals (CIs) at the freedom degree of 50, indicating the chemometrically estimated relative SD (RSD) of a peak area and RSD by repeated measurements of at least 50 times had equivalent reliability.
一种噪声滤波器，通常连接到色谱仪的检测器上，用于提高色谱图的信噪比（S/N）。本文的目的是通过互信息（FUMI）理论阐明在超高效液相色谱（UHPLC）的紫外检测器中噪声滤波对化学计量学评估重复性的统计可靠性的影响。为了检验与噪声滤波相关的 UHPLC 系统中化学计量学评估重复性的统计可靠性，从具有噪声滤波的六个色谱图中获得了峰区 k 处基线波动的面积的标准偏差（SD）值（s（k））。此外，从 s（k）值（n=6）中计算 s（k）值的平均值（σ̂），并将其作为总体 SD 进行替代应用。所有 s（k）/σ̂值在自由度为 50 时均在 95%置信区间（CI）内，表明峰面积的化学计量学估计相对 SD（RSD）和至少 50 次重复测量的 RSD 具有等效的可靠性。
