Department of Petroleum Engineering, Faculty of Petroleum and Chemical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Department of Microbial Biotechnology, School of Biology, College of Science, University of Tehran, Tehran, Iran.
Sci Rep. 2024 Sep 4;14(1):20645. doi: 10.1038/s41598-024-71513-0.
The accumulation of nisin in the fermentation medium can reduce the process's productivity. This research studied the potential of Nymphaea alba leaf powder (NALP) as a hydrophobic biosorbent for efficient in-situ nisin adsorption from the fermentation medium by docking and experimental analysis. Molecular docking analysis showed that di-galloyl ellagic acid, a phytochemical compound found in N. alba, had the highest affinity towards nisin. Enhancements in nisin adsorption were seen following pre-treatment of NAPL with HCl and MgCl. A logistic growth model was employed to evaluate the growth dynamics of the biosorption capacity, offering valuable insights for process scalability. Furthermore, optimization through Response Surface Methodology elucidated optimal nisin desorption conditions by Liebig's law of the minimum, which posits that the scarcest resource governs production efficiency. Fourier Transform Infrared (FTIR) spectroscopy pinpointed vital functional groups involved in biosorption. Scanning electron microscopy revealed the changing physical characteristics of the biosorbent after exposure to nisin. The findings designate NALP as a feasible adsorbent for nisin removal from the fermentation broth, thus facilitating its application in the purification of other biotechnological products based on growth and production optimization principles.
发酵培养基中乳链菌肽的积累会降低生产效率。本研究通过对接和实验分析，研究了睡莲叶粉（NALP）作为一种疏水性生物吸附剂，从发酵培养基中高效原位吸附乳链菌肽的潜力。分子对接分析表明，在 N. alba 中发现的二没食子酰基鞣花酸对乳链菌肽具有最高的亲和力。用 HCl 和 MgCl 预处理 NAPL 后，观察到乳链菌肽的吸附增强。逻辑增长模型用于评估生物吸附容量的生长动态，为工艺可扩展性提供了有价值的见解。此外，通过最小限制律的响应面方法论优化确定了最佳的乳链菌肽解吸条件，该定律认为最稀缺的资源决定了生产效率。傅里叶变换红外（FTIR）光谱指出了生物吸附中涉及的重要功能基团。扫描电子显微镜显示了暴露于乳链菌肽后生物吸附剂物理特性的变化。研究结果表明，NALP 是一种可行的乳链菌肽去除剂，可用于发酵液中其他生物技术产品的纯化，基于生长和生产优化原则。
