Mohamed Fatma K, Ahmed Amal A M, El-Baz Amany A, Galhom Rania A, Osman Abdel-Hamid K
Department of Cytology and Histology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt.
Department of Physiology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.
Tissue Cell. 2024 Dec;91:102543. doi: 10.1016/j.tice.2024.102543. Epub 2024 Aug 30.
Diabetic peripheral neuropathy (DPN) is a common complication of diabetes that affects the angiogenesis and myelination of peripheral nerves. In this study, we investigated the potential of mesenchymal stem cells (MSCs) transplantation to improve DPN by enhancing angiogenesis and remyelination in the sciatic nerve of streptozotocin (STZ)-induced diabetic female rats. The purpose of this study was to evaluate the therapeutic potential of mesenchymal stem cells as a possiblity for clinical intervention to alleviate the symptoms of diabetic peripheral neuropathy. We examined whether transplanted mesenchymal stem cells can produce new and restored angiogenesis, as well as promoting myelination. Overall, our findings suggest that MSCs transplantation has neuroprotective effects. This is particularly the case for Schwann cells. Transplantation may stimulate angiogenesis as well as remyelination of the sciatic nerve in experimentally-induced diabetic peripheral neuropathy. Behavioral assays, histological analysis, and molecular techniques were used to assess the effects of MSCs transplantation. Our results demonstrate that in diabetic rats signs of neuropathy were reversed following a single administration of bone marrow-derived MSCs. Morphological and morphometric analysis of the sciatic nerve revealed that diabetic rats displayed structural alterations that were attenuated with MSCs transplantation.Immunostaining analysis showed increased expression of S100 and VEGF in the sciatic nerve following MSCs transplantation. Western blotting analysis also revealed elevated levels of VEGF and CD31 in rats treated with MSCs compared to diabetic rats.
糖尿病周围神经病变（DPN）是糖尿病常见的并发症，会影响周围神经的血管生成和髓鞘形成。在本研究中，我们探究了间充质干细胞（MSCs）移植通过增强链脲佐菌素（STZ）诱导的糖尿病雌性大鼠坐骨神经的血管生成和髓鞘再生来改善DPN的潜力。本研究的目的是评估间充质干细胞作为一种临床干预可能性以减轻糖尿病周围神经病变症状的治疗潜力。我们检查了移植的间充质干细胞是否能产生新的并恢复血管生成，以及促进髓鞘再生。总体而言，我们的研究结果表明MSCs移植具有神经保护作用。雪旺细胞尤其如此。移植可能会刺激实验性诱导的糖尿病周围神经病变中坐骨神经的血管生成和髓鞘再生。行为学检测、组织学分析和分子技术被用于评估MSCs移植的效果。我们的结果表明，在糖尿病大鼠单次给予骨髓来源的MSCs后，神经病变体征得到了逆转。坐骨神经的形态学和形态计量学分析表明，糖尿病大鼠表现出的结构改变在MSCs移植后有所减轻。免疫染色分析显示，MSCs移植后坐骨神经中S100和VEGF的表达增加。蛋白质印迹分析还显示，与糖尿病大鼠相比，接受MSCs治疗的大鼠中VEGF和CD31的水平升高。
