Tseng L, Bellino F L
J Steroid Biochem. 1985 Apr;22(4):555-7. doi: 10.1016/0022-4731(85)90177-3.
The cross-reactivity of human placental microsomal NADPH-cytochrome c reductase antiserum, REDFBIV, against the endometrial reductase alone and as a component of the endometrial aromatase was investigated. Human endometrial particulate fractions were incubated with various amounts of REDFBIV for 1 h at 4 degrees C and both enzyme activities were measured at the end of incubation. The extent of inhibition of these endometrial enzymes was compared with the ability of this antiserum to inhibit the placental microsomal reductase and aromatase activities. The antiserum effectively inhibited the activities of both enzymes in both tissues in a dose dependent manner with aromatase activity inhibited to a greater extent than reductase activity. These results indicate the antiserum to the placental microsomal NADPH-cytochrome c reductase component of aromatase recognizes the reductase component of the aromatase enzyme system in endometrium.
研究了人胎盘微粒体NADPH-细胞色素c还原酶抗血清REDFBIV对单独的子宫内膜还原酶以及作为子宫内膜芳香化酶组分的还原酶的交叉反应性。将人子宫内膜微粒体部分与不同量的REDFBIV在4℃下孵育1小时，并在孵育结束时测量两种酶的活性。将这些子宫内膜酶的抑制程度与该抗血清抑制胎盘微粒体还原酶和芳香化酶活性的能力进行比较。该抗血清以剂量依赖性方式有效抑制两种组织中两种酶的活性，其中芳香化酶活性的抑制程度大于还原酶活性。这些结果表明，针对芳香化酶的胎盘微粒体NADPH-细胞色素c还原酶组分的抗血清识别子宫内膜中芳香化酶系统的还原酶组分。
