Schneider E, Ihle J N, Dy M
Lymphokine Res. 1985 Spring;4(2):95-102.
Incubation of murine spleen and bone marrow cells with homogeneously purified interleukin 3 (IL-3) results in increased urea and ornithine production. This phenomenon is explained by a marked enhancement of arginase activity, assessed by transformation of labeled arginine into labeled urea during culture and by intracellular arginase assay. The enhancement of enzymatic activity is evident after 12 hours of incubation with IL-3, reaching a maximum after 24 hours. It is (1) dose dependent, (2) restricted to cells from hematopoietic organs (i.e., spleen and bone marrow), and (3) independent of cell proliferation, since irradiation of bone marrow cells does not abolish the arginase enhancing effect of IL-3 in spite of complete inhibition of proliferation. Furthermore, this new activity is specific to IL-3, since other colony-stimulating factors, i.e., granulocyte-macrophage colony-stimulating factor (GM-CSF) and L cell-derived colony-stimulating factor (L-CSF), do not induce it.
用高度纯化的白细胞介素3（IL-3）孵育小鼠脾脏和骨髓细胞会导致尿素和鸟氨酸生成增加。这种现象可通过在培养过程中标记精氨酸转化为标记尿素以及细胞内精氨酸酶测定来评估的精氨酸酶活性显著增强来解释。用IL-3孵育12小时后酶活性增强明显，24小时后达到最大值。它具有以下特点：（1）剂量依赖性；（2）仅限于造血器官（即脾脏和骨髓）的细胞；（3）与细胞增殖无关，因为尽管骨髓细胞增殖被完全抑制，但照射骨髓细胞并不会消除IL-3对精氨酸酶的增强作用。此外，这种新活性对IL-3具有特异性，因为其他集落刺激因子，即粒细胞-巨噬细胞集落刺激因子（GM-CSF）和L细胞衍生的集落刺激因子（L-CSF）不会诱导这种活性。
