de Eugenio Laura I, de la Torre Isabel, de Salas Felipe, Vila Francisco, Alonso David, Prieto Alicia, Martínez María Jesús
Centro de Investigaciones Biológicas Margarita Salas Spanish National Research Council (CIB, CSIC) Madrid Spain.
Instituto de Catálisis y Petroleoquímica Spanish National Research Council (ICP, CSIC) Madrid Spain.
Eng Life Sci. 2024 Jul 11;24(9):e202400029. doi: 10.1002/elsc.202400029. eCollection 2024 Sep.
Lignocellulosic biomass, the most abundant natural resource on earth, can be used for cellulosic ethanol production but requires a pretreatment to improve enzyme access to the polymeric sugars while obtaining value from the other components. γ-Valerolactone (GVL) is a promising candidate for biomass pretreatment since it is renewable and bio-based. In the present work, the effect of a pretreatment based on GVL on the enzymatic saccharification of white birch was evaluated at a laboratory scale and the importance of the washing procedure for the subsequent saccharification was demonstrated. Both the saccharification yield and the production of cellulosic ethanol were higher using a noncommercial enzyme crude from than with the commercial cocktail Cellic CTec2 from Novozymes. Furthermore, the production of extracellular cellulases by has been optimized in 2 L bioreactors, with improvements ranging from 40% to 75%. Finally, it was corroborated by isoelectric focus that optimization of cellulase secretion by did not affect the pattern production of the main β-glucosidases and endoglucanases secreted by this fungus.
木质纤维素生物质是地球上最丰富的自然资源，可用于生产纤维素乙醇，但需要进行预处理，以提高酶对聚合糖的可及性，同时从其他成分中获取价值。γ-戊内酯（GVL）是生物质预处理的一个有前景的候选物，因为它是可再生的且基于生物。在本研究中，在实验室规模下评估了基于GVL的预处理对白桦木酶促糖化的影响，并证明了洗涤程序对后续糖化的重要性。使用来自[具体来源未明确]的非商业酶粗提物时，糖化产率和纤维素乙醇产量均高于使用诺维信公司的商业酶制剂Cellic CTec2。此外，[具体真菌未明确]在2 L生物反应器中对胞外纤维素酶的生产进行了优化，产量提高了40%至75%。最后，通过等电聚焦证实，[具体真菌未明确]纤维素酶分泌的优化并未影响该真菌分泌的主要β-葡萄糖苷酶和内切葡聚糖酶的模式产量。
