Pizzorno Paulina, Falchi Lucía, Mañay Nelly, Pistón Mariela, Bühl Valery
Centro Especializado en Química Toxicológica (CEQUIMTOX), Toxicology Area, DEC, Facultad de Química, Universidad de la República, Gral. Flores 2124, Montevideo, Uruguay.
Grupo de Bioanalítica y Especiación (BIOESP), Analytical Chemistry Area, Facultad de Química, Universidad de la República, Gral. Flores 2124, Montevideo, Uruguay.
MethodsX. 2024 Aug 6;13:102893. doi: 10.1016/j.mex.2024.102893. eCollection 2024 Dec.
An analytical method for the determination of toxicological relevant species of arsenic in urine was developed and validated using hydride generation microwave-induced emission spectrometry (HG-MP-AES). This strategy can be used as an alternative to HG-HPLC-ICP-MS considered as a reference technique for arsenic speciation. This procedure is notably less expensive than other techniques and sample preparation and requires only a few steps.•Hydride generation with MP-AES detection has proven to be an effective technique for measuring arsenic metabolites in urine, which is relevant for occupational monitoring and health risk assessment purposes.•This method offers simplicity and cost-effectiveness, serving as an alternative to classical analytical procedures typically used for arsenic analysis in urine.•The methodology has been successfully applied for the purpose of workers' health surveillance.
采用氢化物发生-微波诱导发射光谱法（HG-MP-AES）建立并验证了一种测定尿液中具有毒理学相关性的砷物种的分析方法。该方法可作为HG-HPLC-ICP-MS的替代方法，HG-HPLC-ICP-MS被视为砷形态分析的参考技术。该方法明显比其他技术成本更低，样品制备只需几步。
• 采用MP-AES检测的氢化物发生法已被证明是一种测定尿液中砷代谢物的有效技术，这对于职业监测和健康风险评估具有重要意义。
• 该方法具有简便性和成本效益，可替代通常用于尿液中砷分析的传统分析方法。
• 该方法已成功应用于工人健康监测。
