Hamidon Nurul Hammizah, Dona Anjana Chamilka Thuduhenage, Zin Nik Nor Imam Nik Mat, Nordin Nurul Izza, Sulaiman Shaida Fariza, Abu-Bakar Nurhidanatasha
School of Health Sciences, Universiti Sains Malaysia, Health Campus, 16150 Kubang Kerian, Kelantan, Malaysia.
SIRIM BERHAD, Institute of Biotechnology Research Centre, Block 19, No. 1, Persiaran Dato Menteri, Section 2, 40700 Shah Alam, Selangor, Malaysia.
Trop Life Sci Res. 2024 Jul;35(2):167-185. doi: 10.21315/tlsr2024.35.2.8. Epub 2024 Jul 31.
The antimalarial properties of crude extracts from galls were investigated through bioassay-guided fractionation. Acetone (QIA) and methanol (QIM) crude extracts have been reported to have promising antimalarial activity against (3D7 strain). These extracts were subjected to fractionation using automated preparative high-performance liquid chromatography (prep-HPLC) to identify the most active fractions. Nine fractions were isolated from each extract, of which the fractions QIA11 and QIM16 showed antimalarial activity, with IC values of 17.65 ± 1.82 μg/mL and 24.21 ± 1.88 μg/mL, respectively. In comparison, the standard antimalarial drug artemisinin has an IC value of 0.004 ± 0.001 μg/mL). Through high-resolution liquid chromatography coupled with mass spectrometry (HR-LCMS) analysis of the fractions, four known compounds were successfully identified: gallic acid, ellagic acid, 1,3,6-tris-o-(3,4,5-trihydroxybenzoyl)-beta-d-glucose and 1-O,6-O-digalloyl-beta-D-glucose.
通过生物测定引导的分级分离法研究了五倍子粗提物的抗疟特性。据报道，丙酮（QIA）和甲醇（QIM）粗提物对（3D7株）具有良好的抗疟活性。对这些提取物进行自动制备高效液相色谱（prep-HPLC）分级分离，以鉴定活性最强的馏分。从每种提取物中分离出9个馏分，其中馏分QIA11和QIM16表现出抗疟活性，IC值分别为17.65±1.82μg/mL和24.21±1.88μg/mL。相比之下，标准抗疟药物青蒿素的IC值为0.004±0.001μg/mL）。通过对这些馏分进行高分辨率液相色谱-质谱联用（HR-LCMS）分析，成功鉴定出4种已知化合物：没食子酸、鞣花酸、1,3,6-三-o-（3,4,5-三羟基苯甲酰基）-β-d-葡萄糖和1-O,6-O-二没食子酰基-β-D-葡萄糖。
