Wang Kai, Gu Ke-Feng, Cao Jie, Yang Yu-Shun, Zhu Hai-Liang, Shang Jin-Hui, Zhou Jie-Li
Affiliated Children's Hospital of Jiangnan University, Wuxi 214023, China.
State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, China.
ACS Sens. 2024 Sep 27;9(9):4898-4905. doi: 10.1021/acssensors.4c01490. Epub 2024 Sep 5.
Copper ions, implicated in processes such as oxidative stress and inflammation, are believed to play a crucial role in cardiovascular disease, a prevalent and deadly disease. Despite this, current diagnostic methods fail to detect early stage cardiovascular disease or track copper ion accumulation, limiting our understanding of the disease's progression. Therefore, the development of noninvasive techniques to image copper ions in cardiovascular disease is urgently needed to enhance diagnostic precision and therapeutic strategies. In this study, we report the successful synthesis and application of a copper ion-activated photoacoustic probe, , which exhibits high sensitivity and selectivity toward copper ions both in vitro and in vivo. was able to noninvasively monitor the changes in copper ion levels and differentiate between different mice based on copper ions in urine. Furthermore, the probe demonstrated good photoacoustic stability and exhibited no significant toxicity in the mice. These findings suggest that could be a promising tool for early detection and monitoring of Cu levels in vivo and urine, providing a new perspective on the role of copper ions in cardiovascular disease.
铜离子与氧化应激和炎症等过程有关，被认为在心血管疾病（一种常见且致命的疾病）中起着关键作用。尽管如此，目前的诊断方法无法检测到早期心血管疾病或追踪铜离子的积累，这限制了我们对该疾病进展的理解。因此，迫切需要开发用于心血管疾病中铜离子成像的非侵入性技术，以提高诊断精度和治疗策略。在本研究中，我们报告了一种铜离子激活的光声探针的成功合成与应用，该探针在体外和体内对铜离子均表现出高灵敏度和选择性。该探针能够非侵入性地监测铜离子水平的变化，并根据尿液中的铜离子区分不同的小鼠。此外，该探针表现出良好的光声稳定性，并且在小鼠中未表现出明显的毒性。这些发现表明，该探针可能是体内和尿液中铜离子早期检测和监测的有前途的工具，为铜离子在心血管疾病中的作用提供了新的视角。
