School of Food Science and Engineering, Hainan University, Haikou 570228, PR China.
School of Food Science and Engineering, Hainan University, Haikou 570228, PR China; Collaborative Innovation Center of Nanfan and High-Efficiency Tropical Agriculture, Hainan University, Haikou 570228, China.
Food Chem. 2025 Jan 15;463(Pt 1):141093. doi: 10.1016/j.foodchem.2024.141093. Epub 2024 Aug 31.
High susceptibility of mangoes to low temperature leads to ripening failure that restricts the marketability of products. This study investigated the effect of methyl jasmonate (MeJA) on ripening disorder and mechanism involved in mangoes during refrigeration. Results showed that 50 μM MeJA ameliorated ripening disorder, as indicated by accelerated advancement of ripening-related parameters. Transcriptome analysis revealed that 17,414 significantly differentially expressed genes were mainly enriched in ethylene synthesis, cell wall degradation, starch degradation and sugar transport. Moreover, 8 AP2/ERF transcription factors and 12 ripening-related genes were characterized via qRT-PCR. Afterwards, through the analysis of transcription factor binding sites and cis-acting elements, a regulatory network of ERFs mediated alleviation of ripening disorder conferred by MeJA was constructed. Finally, the interactions between MiERFs and the promoters of target genes were verified by yeast one-hybrid assay. Our findings provide a theoretical basis for improving cold tolerance via counteracting ripening disorder in mangoes.
芒果对低温的高敏感性导致成熟失败，从而限制了产品的市场销售性。本研究探讨了茉莉酸甲酯（MeJA）对冷藏过程中芒果成熟障碍及相关机制的影响。结果表明，50μM MeJA 可改善成熟障碍，表现为成熟相关参数的加速推进。转录组分析表明，17414 个显著差异表达基因主要富集在乙烯合成、细胞壁降解、淀粉降解和糖转运中。此外，通过 qRT-PCR 鉴定了 8 个 AP2/ERF 转录因子和 12 个成熟相关基因。随后，通过转录因子结合位点和顺式作用元件分析，构建了 MeJA 介导的 ERFs 缓解成熟障碍的调控网络。最后，通过酵母单杂交试验验证了 MiERFs 与靶基因启动子之间的相互作用。本研究为通过拮抗芒果成熟障碍来提高其耐寒性提供了理论依据。
