School of Environment and Energy, South China University of Technology, Guangzhou 510006, Guangdong, China.
School of Environment and Energy, South China University of Technology, Guangzhou 510006, Guangdong, China; Key Lab Pollution Control & Ecosystem Restoration in Industry Cluster, Ministry of Education, Guangzhou 510006, Guangdong, China; Guangdong Provincial Key Laboratory of Solid Wastes Pollution Control and Recycling, Guangzhou 510006, Guangdong, China; Guangdong Provincial Engineering and Technology Research Center for Environment Risk Prevention and Emergency Disposal, South China University of Technology, Guangzhou 510006, Guangdong, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2024 Oct 1;1246:124293. doi: 10.1016/j.jchromb.2024.124293. Epub 2024 Aug 31.
This work for the first time reported the complete transformation of 17β-estradiol (E2) to estrone (E1) by unknown wild-type enzyme present in the widely used commercial arylsulfatase derived from Helix pomatia. It was found that acetate could effectively inhibit the unknown enzyme with a half inhibitory concentration (IC) of 140.9 μM, while phosphate and citrate showed no inhibition. Since the buffer solutions with phosphate and citrate have been used in the enzymatic hydrolysis of natural estrogen conjugates for decades, the transformation of E2 to E1 likely occurred during such procedure, inevitably leading to overestimated E1, but underestimated E2. It was further suggested that acetate should be used to prevent this undesirable transformation during the enzymatic hydrolysis of natural estrogen conjugates.
这项工作首次报道了 17β-雌二醇（E2）在广泛应用于商业海兔来源的芳基硫酸酯酶中的未知野生型酶的作用下完全转化为雌酮（E1）。研究发现，乙酸盐能有效抑制该未知酶，其半数抑制浓度（IC）为 140.9 μM，而磷酸盐和柠檬酸盐则没有抑制作用。由于磷酸盐和柠檬酸盐缓冲液已经在天然雌激素结合物的酶水解中使用了几十年，因此 E2 向 E1 的转化很可能发生在这个过程中，这不可避免地导致了对 E1 的高估，但对 E2 的低估。进一步的研究表明，在天然雌激素结合物的酶水解过程中，应该使用乙酸盐来防止这种不理想的转化。
