Binnington K C
Tissue Cell. 1985;17(1):131-40. doi: 10.1016/0040-8166(85)90021-7.
The ultrastructural effects on larval cuticle of Lucilia cuprina of two inhibitors of chitin synthesis, diflubenzuron and polyoxin D and an inhibitor of dihydrofolate reductase, aminopterin, are compared with those of the insecticide, cyromazine. Diflubenzuron and polyoxin D both prevent the formation of a normal lamellate appearance in procuticle and interfere with deposition of epicuticle. Aminopterin and cyromazine cause necrotic lesions in the cuticle which, in the case of cyromazine, are contiguous with invasive processes of epidermal cells. There is an accumulation of electron-dense granules in some epidermal cells in larvae poisoned with aminopterin or cyromazine. Aminopterin has a more drastic cytotoxic effect than cyromazine and it also interferes with the formation of epicuticle. The lesions produced by cyromazine treatment are not mimicked precisely by any of the other chemicals. However, there is closer accord between the effects of cyromazine and aminopterin than between cyromazine and the inhibitors of chitin formation.
将两种几丁质合成抑制剂（二氟苯脲和多氧霉素D）、一种二氢叶酸还原酶抑制剂（氨蝶呤）以及杀虫剂环丙氨嗪对铜绿蝇幼虫表皮的超微结构影响，与杀虫剂环丙氨嗪的影响进行了比较。二氟苯脲和多氧霉素D均会阻止前表皮形成正常的层状外观，并干扰上表皮的沉积。氨蝶呤和环丙氨嗪会导致表皮出现坏死性病变，就环丙氨嗪而言，这些病变与表皮细胞的侵入过程相邻。在用氨蝶呤或环丙氨嗪处理的幼虫中，一些表皮细胞中会积累电子致密颗粒。氨蝶呤比环丙氨嗪具有更强烈的细胞毒性作用，并且它还会干扰上表皮的形成。环丙氨嗪处理产生的病变无法被其他任何一种化学物质精确模拟。然而，环丙氨嗪与氨蝶呤的作用之间的一致性比环丙氨嗪与几丁质形成抑制剂之间的一致性更高。
