State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Collaborative Innovation Center of Food Safety and Quality Control, Jiangnan University, Wuxi, Jiangsu 214122, China.
Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu 214122, China.
J Hazard Mater. 2024 Nov 5;479:135573. doi: 10.1016/j.jhazmat.2024.135573. Epub 2024 Aug 30.
Fusarium graminearum, the primary pathogen responsible for wheat Fusarium head blight, can induce pulmonary damage through its spores. However, the detailed mechanism by which these spores cause intestinal injury is not yet fully understood. This study aimed to investigate the impact of exposure to fungal spores on the intestinal microbiota using a mice model that mimics the effects of fusarium graminearum spores on the gut microbiota and its metabolic profile. The study utilized 16S rRNA sequencing and metabolomics methodologies to analyze the contents of the cecum and feces in mice. The results showed that exposure to fungal spores led to significant changes in the composition of the intestinal microbiota in mice, characterized by an increase in Akkermansia and Staphylococcus populations. A non-targeted metabolomics analysis identified 316 metabolites associated with various metabolic pathways, particularly galactose metabolism. Pre-exposure to antibiotics before fungal spore exposure resulted in a decrease in the metabolic capacity of the intestinal microbiota in mice. This research demonstrates that fusarium graminearum spores can disrupt the intestinal microbiota and metabolome via the lung-gut axis. These findings provide valuable insights into the intestinal damage caused by fungal spores and offer important support for the development of therapeutic strategies for intestinal diseases.
镰刀菌（Fusarium graminearum）是引起小麦赤霉病的主要病原体，其孢子可引发肺部损伤。然而，这些孢子引起肠道损伤的详细机制尚不完全清楚。本研究旨在通过模拟镰刀菌孢子对肠道微生物群及其代谢谱的影响的小鼠模型，研究暴露于真菌孢子对肠道微生物群的影响。该研究利用 16S rRNA 测序和代谢组学方法分析了小鼠盲肠和粪便中的内容物。结果表明，暴露于真菌孢子会导致小鼠肠道微生物群组成发生显著变化，特征是 Akkermansia 和葡萄球菌属种群增加。非靶向代谢组学分析鉴定出与各种代谢途径相关的 316 种代谢物，特别是半乳糖代谢。在真菌孢子暴露前预先暴露于抗生素会导致小鼠肠道微生物群的代谢能力下降。本研究表明，镰刀菌孢子可通过肺-肠轴破坏肠道微生物群和代谢组。这些发现为真菌孢子引起的肠道损伤提供了有价值的见解，并为肠道疾病的治疗策略的发展提供了重要支持。
